Capture and alignment of phi29 viral particles in sub-40 nanometer porous alumina membranes.
Capture and alignment of phi29 viral particles in sub-40 nanometer porous alumina membranes.
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DOI：
10.1007/s10544-008-9217-0
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发表时间：
2009-02
影响因子：
2.8
通讯作者：
Bashir R
中科院分区：
文献类型：
--
作者：
Moon JM;Akin D;Xuan Y;Ye PD;Guo P;Bashir R
噬菌体phi29病毒纳米颗粒及其相关的DNA包装纳米颗粒可以为开发混合生物纳米结构提供新的可能性。为了将phi29病毒和纳米发动机与人工微米和纳米结构连接起来，我们制造了孔径为70 nm的纳米多孔阳极氧化铝（AAO）膜，并使用氧化铝的原子层沉积（ALD）将孔缩小到40 nm以下。我们能够使用两种方法捕获和排列阳极氧化纳米孔中的颗粒。首先，开发了将颗粒化学附着在孔的内表面中的功能化和抛光工艺。其次，利用颗粒的离心将它们排列在纳米多孔膜的孔中。此外，当空衣壳和包装颗粒的混合物以特定速度离心时，发现空衣壳变形并穿过40 nm直径的孔，而包装有DNA的颗粒主要保留在纳米多孔膜的顶面。使用荧光显微术来验证空衣壳通过纳米多孔膜的选择性过滤。
Bacteriophage phi29 virus nanoparticles and its associated DNA packaging nanomotor can provide for novel possibilities towards the development of hybrid bio-nano structures. Towards the goal of interfacing the phi29 viruses and nanomotors with artificial micro and nano-structures, we fabricated nanoporous Anodic Aluminum Oxide (AAO) membranes with pore size of 70 nm and shrunk the pores to sub 40 nm diameter using atomic layer deposition (ALD) of Aluminum Oxide. We were able to capture and align particles in the anodized nanopores using two methods. Firstly, a functionalization and polishing process to chemically attach the particles in the inner surface of the pores was developed. Secondly, centrifugation of the particles was utilized to align them in the pores of the nanoporous membranes. In addition, when a mixture of empty capsids and packaged particles was centrifuged at specific speeds, it was found that the empty capsids deform and pass through 40 nm diameter pores whereas the particles packaged with DNA were mainly retained at the top surface of the nanoporous membranes. Fluorescence microscopy was used to verify the selective filtration of empty capsids through the nanoporous membranes.
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