Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd(2+)).
Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd(2+)).
复制标题
接触重金属镉 (Cd2) 的罗氏沼虾鳃和肝胰腺的比较转录组分析
DOI：
10.1038/s41598-021-95709-w
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发表时间：
2021-08-09
影响因子：
4.6
通讯作者：
Ma K
中科院分区：
文献类型：
--
作者：
Liu X;Jiang H;Ye B;Qian H;Guo Z;Bai H;Gong J;Feng J;Ma K
Heavy metal Cadmium (Cd2+) pollution has become a severe environmental problem for aquatic organisms. In crustaceans, gills (Gi) and hepatopancreas (Hp) play a vital role in the toxicology. However, in Macrobrachium rosenbergill, there are few researches about gill and hepatopancreases responding to Cd2+ stress at a molecular level. In this study, transcriptomic analysis was applied to characterize gene expression profiles of gills and hepatopancreas of M. rosenbergill after Cd2+ exposure for 0 h, 3 h and 3 d. Six cDNA libraries (Gi 0 h, Gi 3 h, Gi 3 d, Hp 0 h, Hp 3 h, and Hp 3 d) were constructed and a total of 66,676 transcripts and 48,991 unigenes were annotated. Furthermore, differentially expressed genes (DEGs) were isolated by comparing the Cd2+ treated time-point libraries (3 h and 3 d group) with the control library (0 h group). The results showed that most of the DEGs were down-regulated after Cd2+ exposure and the number of DEGs among gill groups were significantly higher than those among hepatopancreas groups. GO functional and KEGG pathway analysis suggested many key DEGs in response to the Cd2+ stress, such as metallothionein and Hemocyanin. Additionally, a total of six DEGs were randomly selected to further identify their expressional profile by qPCR. The results indicated that these DEGs were involved in the response to Cd2+. This comparative transcriptome provides valuable molecular information on the mechanisms of responding to Cd2+ stress in M. rosenbergii, which lays the foundation for further understanding of heavy metal stress.
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影响因子：
14.9
作者：
Kanehisa M;Furumichi M;Sato Y;Ishiguro-Watanabe M;Tanabe M
通讯作者：
Tanabe M
DOI：
10.1007/s00254-001-0445-3
发表时间：
2002-03-01
期刊：
ENVIRONMENTAL GEOLOGY
影响因子：
--
作者：
Borrego, J;Morales, JA;Grande, JA
通讯作者：
Grande, JA
DOI：
10.1016/0010-406x(70)90369-5
发表时间：
1970-01-01
期刊：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY
影响因子：
--
作者：
HOCHACHKA, PW;SOMERO, GN;FREED, JM
通讯作者：
FREED, JM
影响因子：
12.8
作者：
Gaspic, ZK;Zvonaric, T;Baric, A
通讯作者：
Baric, A
影响因子：
8.8
作者：
Guo, Hui;Deng, Mingyue;Shen, Yuchun
通讯作者：
Shen, Yuchun