PCR Primers for Microsatellite Loci in Tuna Species of the Genus Thunnus and its Application for Population Genetic Study
PCR Primers for Microsatellite Loci in Tuna Species of the Genus Thunnus and its Application for Population Genetic Study
复制标题
金枪鱼微卫星位点PCR引物及其在群体遗传研究中的应用
DOI：
--
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
N. Taniguchi
中科院分区：
文献类型：
--
作者：
M. Takagi;T. Okamura;S. Chow;N. Taniguchi
从太平洋北部金枪鱼（Thun nus thynnus orientalis）基因组文库中分离出微卫星位点，设计了扩增4个位点的PCR引物。对35 ~ 40份太平洋北部蓝鳍金枪鱼样本进行遗传多态性分析，结果表明，这些位点均存在高度的长度多态性，等位基因数在8 ~ 23个之间，杂合度在0.533 ~ 1之间。将这些引物组合应用于大西洋北部蓝鳍金枪鱼T. T. thynnus、长鳍金枪鱼T. alalunga、大眼金枪鱼T. obesus和黄鳍金枪鱼T. albacares，检测到所有位点的多态性与太平洋北部蓝鳍金枪鱼的多态性相当。在太平洋和大西洋北部蓝鳍金枪鱼样本中观察到等位基因频率的显著差异。这些为太平洋北部蓝鳍金枪鱼开发的引物似乎可用于扩增其他金枪鱼物种的同源微卫星位点，并且可能具有作为金枪鱼属金枪鱼物种内部和样本之间遗传变异指标的巨大潜力。
Microsatellite loci were isolated from a size-selected genomic library of Pacific northern tuna Thun nus thynnus orientalis, and PCR primer sets to amplify four loci were designed. Investigation on genet ic polymorphism at these loci in the Pacific northern bluefin tuna sample (n=35-40) revealed high degree of length polymorphisms in all loci, in which number of alleles per locus ranged from 8 to 23 and observed heterozygosity from 0.533 to 1. These primer sets were applied to Atlantic northern bluefin tuna T. t. thynnus, albacore T. alalunga, bigeye tuna T. obesus and yellowfin tuna T. albacares, detect ing polymorphism in all loci comparable with those of Pacific northern bluefin tuna. Significant differ ences in the allele frequency were observed between Pacific and Atlantic northern bluefin tuna samples. These primer sets developed for Pacific northern bluefin tuna appeared to be useful for amplifying homologous microsatellite loci in the other Thunnus tuna species, and may have great potential as indi cators for genetic variability within and between samples of tuna species of the genus Thunnus.
影响因子：
1.9
作者：
GUO, SW;THOMPSON, EA
通讯作者：
THOMPSON, EA