A novel tetratricopeptide repeat protein, WHITE TO GREEN1, is required for early chloroplast development and affects RNA editing in chloroplasts.
A novel tetratricopeptide repeat protein, WHITE TO GREEN1, is required for early chloroplast development and affects RNA editing in chloroplasts.
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DOI：
10.1093/jxb/erx383
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发表时间：
2017-12-16
影响因子：
6.9
通讯作者：
Sodmergen
中科院分区：
文献类型：
--
作者：
Ma F;Hu Y;Ju Y;Jiang Q;Cheng Z;Zhang Q;Sodmergen
WTG1参与早期叶绿体发育，可能通过增强拟南芥中RNA编辑体的功能而发挥RNA编辑功能。叶绿体对于植物光合作用和生产至关重要，但叶绿体发育的调控机制仍不清楚。通过筛选拟南芥叶片着色突变体，发现一个新基因 WHITE TO GREEN1 (WTG1) 在叶绿体发育和质体基因表达中具有功能。 WTG1 编码叶绿体定位的四肽重复蛋白，该蛋白在拟南芥细胞中广泛表达。 WTG1 的破坏会抑制植物生长，延缓叶片绿化和叶绿体发育，并抑制光合基因表达，但 WTG1 的补充表达会恢复正常表型。此外，WTG1蛋白与细胞器RNA编辑因子MORF8和MORF9相关，并且质体petL-5和ndhG-50转录本的RNA编辑在wtg1突变体中受到影响。这些结果表明WTG1影响质体基因表达的转录和转录后调节，并为四三肽重复蛋白参与拟南芥叶绿体RNA编辑提供了证据。
WTG1 is involved in early chloroplast development and may function in RNA editing by enhancing the function of RNA editosomes in Arabidopsis The chloroplast is essential for plant photosynthesis and production, but the regulatory mechanism of chloroplast development is still elusive. Here, a novel gene, WHITE TO GREEN1 (WTG1), was identified to have a function in chloroplast development and plastid gene expression by screening Arabidopsis leaf coloration mutants. WTG1 encodes a chloroplast-localized tetratricopeptide repeat protein that is expressed widely in Arabidopsis cells. Disruption of WTG1 suppresses plant growth, retards leaf greening and chloroplast development, and represses photosynthetic gene expression, but complemented expression of WTG1 restored a normal phenotype. Moreover, WTG1 protein is associated with the organelle RNA editing factors MORF8 and MORF9, and RNA editing of the plastid petL-5 and ndhG-50 transcripts was affected in wtg1 mutants. These results indicate that WTG1 affects both transcriptional and posttranscriptional regulation of plastid gene expression, and provide evidence for the involvement of a tetratricopeptide repeat protein in chloroplast RNA editing in Arabidopsis.
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