Acute effects of splint immobilization of the forearm on in vivo microcirculation and histomorphology of the human skin
Acute effects of splint immobilization of the forearm on in vivo microcirculation and histomorphology of the human skin
复制标题
前臂夹板固定对体内微循环及人体皮肤组织形态的急性影响
DOI：
10.1002/jemt.22317
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发表时间：
2014
影响因子：
2.5
通讯作者：
M. A. Altintas
中科院分区：
文献类型：
--
作者：
A. A. Altintas;P. M. Vogt;M. A. Altintas
背景：临床上经常使用小夹板固定前臂。先前的研究调查了制动对骨、软骨、肌肉和肌腱的影响，然而，对人体皮肤微循环和组织形态学的急性影响仍然是难以捉摸的。方法：在12名健康、不吸烟的个体（年龄29.7 ± 9.1岁）中，随机选择一个前臂通过夹板固定72小时，而另一个前臂作为对照。在动员前（基线值）和动员后进行体内反射模式共聚焦显微镜检查（RMCM），以评价：定量血细胞流量;功能性真皮毛细血管密度;表皮厚度;和颗粒细胞大小。研究结果：前臂制动72小时，定量血细胞流量显著减少（42.86 ± 3.68个细胞/min），与对照血流相比（53.11 ± 3.68个/min，P < 0.05），真皮毛细血管功能密度较低（5.73 ± 0.63）个毛细血管/mm ~ 2，对照组（7.04 ± 0.81）个毛细血管/mm ~ 2，P < 0.05。组织学评估显示，与对照部位相比，制动后表皮明显更薄（40.02 ± 2.91 µ m vs. 46.64±3.09 µm，P < 0.05）。与770.2 ± 38.21 µm2的对照细胞大小相比，72 h夹板固定时颗粒细胞大小（730.1 ± 42.53 µm2）发生显著变化。两前臂基线值比较，各参数均无统计学意义（P > 0.05）。结论：在72小时夹板固定，第一次，显着的适应机制进行了评估，对人体皮肤微循环和组织形态学在体内RMCM。这些适应性变化可以被认为是人类皮肤的初期萎缩。应在进一步的RMCM研究中评估制动的长期影响，包括再生潜力。Microsc. Res. Tech. 77：99-103，2014.© 2013威利期刊公司.
BACKGROUND: Splint immobilization of the forearm is often performed in clinical practice. Previous studies investigated the effect of immobilization on bone, cartilage, muscle, and tendon, however, the acute effects on human skin microcirculation and histomorphology remains elusive. METHODS: In 12 healthy, nonsmoking individuals (aged 29.7 ± 9.1 years) a randomly selected forearm was immobilized by splinting for 72 h, whereas the other forearm served as control. In vivo Reflectance‐Mode Confocal‐Microscopy (RMCM) was performed prior (baseline value) and postimmobilization to evaluate: quantitative blood cell flow; density of functional dermal capillaries; epidermal thickness; and granular cell size. RESULTS: At 72h forearm immobilization, quantitative blood cell flow was significantly reduced (42.86 ± 3.68 cells/min) compared to the control blood flow (53.11 ± 3.68 cells/min, P < 0.05) and dermal capillaries indicates less functional density (5.73 ± 0.63 capillaries/mm2) compared to the controls (7.04 ± 0.81 capillaries/mm2, P < 0.05). Histometric assessment reveals significantly thinner epidermis following immobilization compared to the control site (40.02 ± 2.91 vs. 46.64±3.09 µm, P < 0.05). Granular cell size was significantly altered at 72 h splinting (730.1 ± 42.53 µm2) compared to the control cell size at 770.2 ± 38.21 µm2. Comparison of baseline values of both forearms indicate statistically insignificance (P > 0.05) for each parameter. CONCLUSION: At 72 h splint immobilization, for the first time, significant adaptive mechanisms were evaluated on human skin microcirculation and histomorphology using in vivo RMCM. These adaptations may be considered as an incipient atrophy of the human skin. Long‐term effects of immobilization including the regenerative potential should be evaluated in further RMCM studies. Microsc. Res. Tech. 77:99–103, 2014. © 2013 Wiley Periodicals, Inc.
皮肤病学中的体内反射模式共焦扫描激光显微镜。
DOI：
--
发表时间：
2004
期刊：
Advances in dermatology
影响因子：
--
作者：
Gonzalez,Salvador;Gilaberte-Calzada,Yolanda;Gonzalez-Rodriguez,Antonia;Torres,Abel;MihmJr,MartinC
通讯作者：
MihmJr,MartinC
DOI：
10.3109/17453678208992224
发表时间：
1982-01-01
期刊：
ACTA ORTHOPAEDICA SCANDINAVICA
影响因子：
--
作者：
AMIEL, D;WOO, SLY;AKESON, WH
通讯作者：
AKESON, WH