Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers.
Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers.
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DOI：
10.1038/ncb1800
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发表时间：
2008-12
影响因子：
21.3
通讯作者：
Breakefield, Xandra O.
中科院分区：
文献类型：
--
作者：
Skog, Johan;Wuerdinger, Tom;van Rijn, Sjoerd;Meijer, Dimphna H.;Gainche, Laura;Sena-Esteves, Miguel;Curry, William T., Jr.;Carter, Bob S.;Krichevsky, Anna M.;Breakefield, Xandra O.
胶质母细胞瘤肿瘤细胞释放含有mRNA、miRNA和血管生成蛋白的微泡（外来体）。这些微泡被正常宿主细胞，如脑微血管内皮细胞吸收。通过将报告蛋白的mRNA掺入这些微泡中，我们证明了微泡传递的信息由受体细胞翻译。这些微泡还富含血管生成蛋白并刺激内皮细胞形成小管。因此，肿瘤来源的微泡作为一种手段，提供遗传信息和蛋白质的受体细胞在肿瘤环境中。胶质母细胞瘤微泡也刺激人类胶质瘤细胞系的增殖，表明自我促进方面。在胶质母细胞瘤患者的血清微泡中可以检测到胶质瘤特征性的信使RNA突变体/变体和miRNA。在25例胶质母细胞瘤患者中的7例的血清微泡中检测到肿瘤特异性EGFRvIII。因此，肿瘤衍生的微泡可以通过血液测试为癌症患者提供诊断信息并帮助治疗决策。
Glioblastoma tumour cells release microvesicles (exosomes) containing mRNA, miRNA and angiogenic proteins. These microvesicles are taken up by normal host cells, such as brain microvascular endothelial cells. By incorporating an mRNA for a reporter protein into these microvesicles, we demonstrate that messages delivered by microvesicles are translated by recipient cells. These microvesicles are also enriched in angiogenic proteins and stimulate tubule formation by endothelial cells. Tumour-derived microvesicles therefore serve as a means of delivering genetic information and proteins to recipient cells in the tumour environment. Glioblastoma microvesicles also stimulated proliferation of a human glioma cell line, indicating a self-promoting aspect. Messenger RNA mutant/variants and miRNAs characteristic of gliomas could be detected in serum microvesicles of glioblastoma patients. The tumour-specific EGFRvIII was detected in serum microvesicles from 7 out of 25 glioblastoma patients. Thus, tumour-derived microvesicles may provide diagnostic information and aid in therapeutic decisions for cancer patients through a blood test.
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DOI：
10.1073/pnas.0334355100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Mallardo, M;Deitinghoff, A;Kiebler, MA
通讯作者：
Kiebler, MA
影响因子：
4.1
作者：
Rolhion, C;Penault-Llorca, F;Verrelle, P
通讯作者：
Verrelle, P
影响因子：
3.7
作者：
Badr CE;Hewett JW;Breakefield XO;Tannous BA
通讯作者：
Tannous BA
影响因子：
5.7
作者：
Delves, George H.;Stewart, Alistair B.;Lwaleed, Bashir A.
通讯作者：
Lwaleed, Bashir A.
影响因子：
6.4
作者：
Janowska-Wieczorek, A;Wysoczynski, M;Ratajczak, MZ
通讯作者：
Ratajczak, MZ