Factors controlling the time of onset of the migration of neural crest cells in the fowl embryo
Factors controlling the time of onset of the migration of neural crest cells in the fowl embryo
复制标题
控制家禽胚胎中神经嵴细胞迁移开始时间的因素
作者：
D. Newgreen;I. Gibbins
在干水平神经嵴（NC）细胞迁移前7-10小时期间，鸡胚胎的透射电子显微镜显示NC细胞附近的细胞外物质。与神经管细胞相反，NC细胞的基底表面具有突起，并且既不连续也不被完整的基底层覆盖。NC-细胞的顶端区显示在神经折叠融合的阶段的细胞间连接，但这种连接是不存在的一些NC-细胞迁移前5小时。迁移前，神经管和外胚层的基底层在NC的外侧融合。在体外，NC-细胞迁移开始立即当神经原基上富含纤连蛋白的基质，但只有当神经原基的迁移已经开始在体内的水平。迁移延迟4-8小时时，使用premigratory-level澄清剂。短期细胞粘附试验表明，NC-细胞的迁移前和迁移水平可以坚持富含纤维连接蛋白的基质和胶原蛋白凝胶，但只有迁移NC-细胞可以脱离神经原基。结果表明，NC细胞迁移的确切时间表与NC细胞的细胞间粘附的减少相关。
SummaryTransmission electron microscopy of fowl embryos during the 7–10 h preceding migration of trunk-level neural crest (NC) cells revealed extracellular material near the NC-cells. In contrast to the cells of the neural tube, the basal surfaces of NC-cells possessed projections, and were neither contiguous nor covered by a complete basal lamina. The apical zones of NC-cells showed intercellular junctions at the stage of neural-fold fusion, but such junctions were absent in some NC-cells 5 h before migration. The basal laminae of the neural tube and the ectoderm were fused lateral to the NC before migration. In vitro, NC-cell migration commenced immediately when neural anlagen were explanted onto fibronectin-rich matrices, but only when the neural anlagen were from a level where migration had commenced in vivo. Migration was delayed 4–8 h when premigratory-level expiants were used. Short-term cell-adhesion assays showed that NC-cells of both premigratory and migratory levels could adhere to fibronectin-rich matrices and to collagen gels, but only migratory NC-cells could be detached from the neural anlage. The results suggest that the precise schedule of the onset of NC-cell migration correlates with a decrease in the intercellular adhesion of NC-cells.
在存在和不存在血浆纤连蛋白的情况下，成纤维细胞粘附在胶原基质上。
影响因子：
4
作者：
Grinnell,F;Bennett,MH
通讯作者：
Bennett,MH
DOI：
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发表时间：
1981-06
期刊：
Journal of embryology and experimental morphology
影响因子：
--
作者：
E. M. Davis;J. Trinkaus
通讯作者：
E. M. Davis;J. Trinkaus