Seawater is a reservoir of multi-resistant Escherichia coli, including strains hosting plasmid-mediated quinolones resistance and extended-spectrum beta-lactamases genes.
Seawater is a reservoir of multi-resistant Escherichia coli, including strains hosting plasmid-mediated quinolones resistance and extended-spectrum beta-lactamases genes.
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DOI：
10.3389/fmicb.2014.00426
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发表时间：
2014
影响因子：
5.2
通讯作者：
Henriques I
中科院分区：
文献类型：
--
作者：
Alves MS;Pereira A;Araújo SM;Castro BB;Correia AC;Henriques I
本研究的目的是研究抗生素耐药性（AR）在沿海水域的传播，考虑到不同来源的粪便污染的贡献。样本采集于Berklia，这是一个被列为自然保护区的无人居住岛屿，游客经常前往该岛进行水上娱乐活动。为了实现我们的目标，AR大肠杆菌分离株从沿海水域进行了比较，AR分离株从两个来源的粪便污染：人源性污水和海鸥粪便。分离E.大肠杆菌中进行。根据基因分型结果，共鉴定出414株菌株。分布E.大肠杆菌系统发育群在所有来源的分离株之间是相似的。对链霉素、四环素、头孢噻吩和阿莫西林的耐药最常见。在海水和粪便分离株中发现了较高的AR率，除了人类医学中使用的最后一线抗生素。来自污水和海鸥粪便的分离株多重耐药率（29%和32%）低于海水分离株（39%）。海水AR配置文件相似，从海鸥粪便和污水AR配置文件显着不同。在所有来源的分离株中均存在与耐药基因blaTEM、sul 1、sul 2、泰特（A）和泰特（B）匹配的核苷酸序列。在海水（blaCTX-M-1和blaSHV-12）和海鸥粪便（blaCMY-2）中检测到赋予对第三代头孢菌素的抗性的基因。质粒介导的决定因素耐喹诺酮类药物被发现：qnrS 1在所有来源和qnrB 19在海水和海鸥粪便。我们的研究结果表明，海水是AR的相关水库，海鸥是传播人类相关细菌和耐药基因的有效载体。急诊大肠杆菌resistome夺回从BERDERA沿海水域主要是调制海鸥粪便污染。耐药基因库涵盖了对人类至关重要的抗生素，这是对人类健康的潜在风险。
The aim of this study was to examine antibiotic resistance (AR) dissemination in coastal water, considering the contribution of different sources of fecal contamination. Samples were collected in Berlenga, an uninhabited island classified as Natural Reserve and visited by tourists for aquatic recreational activities. To achieve our aim, AR in Escherichia coli isolates from coastal water was compared to AR in isolates from two sources of fecal contamination: human-derived sewage and seagull feces. Isolation of E. coli was done on Chromocult agar. Based on genetic typing 414 strains were established. Distribution of E. coli phylogenetic groups was similar among isolates of all sources. Resistances to streptomycin, tetracycline, cephalothin, and amoxicillin were the most frequent. Higher rates of AR were found among seawater and feces isolates, except for last-line antibiotics used in human medicine. Multi-resistance rates in isolates from sewage and seagull feces (29 and 32%) were lower than in isolates from seawater (39%). Seawater AR profiles were similar to those from seagull feces and differed significantly from sewage AR profiles. Nucleotide sequences matching resistance genes blaTEM, sul1, sul2, tet(A), and tet(B), were present in isolates of all sources. Genes conferring resistance to 3rd generation cephalosporins were detected in seawater (blaCTX-M-1 and blaSHV-12) and seagull feces (blaCMY-2). Plasmid-mediated determinants of resistance to quinolones were found: qnrS1 in all sources and qnrB19 in seawater and seagull feces. Our results show that seawater is a relevant reservoir of AR and that seagulls are an efficient vehicle to spread human-associated bacteria and resistance genes. The E. coli resistome recaptured from Berlenga coastal water was mainly modulated by seagulls-derived fecal pollution. The repertoire of resistance genes covers antibiotics critically important for humans, a potential risk for human health.
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DOI：
10.1007/s10096-013-1983-9
发表时间：
2014-04-01
影响因子：
4.5
作者：
Freitas, F.;Machado, E.;Peixe, L.
通讯作者：
Peixe, L.
影响因子：
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作者：
Carroll, S. P.;Dawes, Les;Goonetilleke, A.
通讯作者：
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影响因子：
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作者：
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Dolejska, M.;Bierosova, B.;Cizek, A.
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Cizek, A.
影响因子：
5.2
作者：
Dallenne, Caroline;Da Costa, Anaelle;Arlet, Guillaume
通讯作者：
Arlet, Guillaume