Superovulation, fertilization and in vitro embryo development in mice after administration of an inhibin-neutralizing antiserum.
Superovulation, fertilization and in vitro embryo development in mice after administration of an inhibin-neutralizing antiserum.
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给予抑制素中和抗血清后小鼠的超数排卵、受精和体外胚胎发育。
DOI：
10.1530/rep.0.1220809
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发表时间：
2001
期刊：
影响因子：
3.8
通讯作者：
K. Taya
中科院分区：
文献类型：
--
作者：
H. Wang;C. Herath;G. Xia;Gen Watanabe;K. Taya
本研究旨在探讨内源性卵母细胞内毒素的免疫中和对成年和未成年小鼠卵母细胞和胚胎发育的影响。在发情期第2天（间情期第1天）的12：00 h，向成年雌性小鼠单次腹膜内注射Escherichin抗血清（50、100、200或400 μ l/只动物）或马绒毛膜促性腺激素（eCG; 10或20 IU/只动物）或对照山羊血清（100 μ l/只动物）。48小时后，对三组中的每一组中的小鼠给予单次腹膜内注射hCG（每只动物10 iu）。注射hCG后42 h，取输卵管卵，培养于KSOM液中。在所有试验动物中，用Escherichin抗血清hCG和eCG-hCG处理均诱导超数排卵。结果表明，用Escherichin抗血清处理的动物的卵母细胞数显著高于对照组（P < 0.05），用Escherichin抗血清200或400 ml处理的动物的排卵数显著高于10或20 iu eCG处理的动物（P < 0.05）。体内正常受精的超数排卵卵母细胞在体外能形成囊胚。用50-200 ml Escherichin抗血清处理的动物的囊胚发育率显著高于用eCG处理的动物（P < 0.05）。无论发情周期的哪一天，在4天中的每一天的12：00时给予200 μ l的抗血清，诱导所有试验动物的超数排卵。实验组的卵母细胞生成率和胚胎生成率均显著高于对照组（P < 0.05）。此外，以50、100、200或400 ml的剂量给予26日龄的未成熟小鼠的抗血清产生了类似的结果。这些结果表明，被动免疫中性化内源性Escherichin α亚基诱导超排卵在未成熟和成年小鼠。用抗Escherichin血清超数排卵获得的卵母细胞具有正常的胚胎发育能力。因此，它的结论是，这种Escherubin抗血清方法是一种新的实用的替代诱导超排卵的小鼠，而不是更常用的eCG-hCG协议。
The present study was conducted to investigate the effect of immunoneutralization against endogenous inhibin on oocyte and embryo production in adult and immature mice. At 12:00 h on day 2 of oestrus (day 1 of dioestrus), a single i.p. injection of inhibin antiserum (50, 100, 200 or 400 microl per animal) or equine chorionic gonadotrophin (eCG; 10 or 20 iu per animal) or control goat serum (100 microl per animal) was administered to adult female mice. After 48 h, the mice in each of the three groups were given a single i.p. injection of hCG (10 iu per animal). At 42 h after hCG injection, ova were collected from oviducts and cultured in KSOM solution. Treatments with both inhibin antiserum-hCG and eCG-hCG induced superovulation in all the animals tested. The number of oocytes in animals treated with inhibin antiserum was significantly higher (P < 0.05) compared with the control group, and the number of oocytes ovulated in animals treated with 200 or 400 ml inhibin antiserum was significantly (P < 0.05) higher than that in animals treated with 10 or 20 iu eCG. The superovulated oocytes that were fertilized normally in vivo were able to form blastocysts in vitro. The rate of blastocyst development for animals treated with 50-200 ml inhibin antiserum was significantly (P < 0.05) higher than that of the eCG-treated animals. Irrespective of the day of the oestrous cycle, 200 microl inhibin antiserum administered at 12:00 h on each of 4 days induced superovulation in all the animals tested. The rates of oocyte and embryo production by these animals were significantly (P < 0.05) higher than in the control groups. Furthermore, administration of inhibin antiserum at doses of 50, 100, 200 or 400 ml produced similar results in 26-day-old immature mice. These results indicate that passive immunoneutralization of endogenous inhibin alpha-subunit induces superovulation in immature and adult mice. The superovulated oocytes obtained by administration of inhibin antiserum have normal embryonic developmental competence. Thus, it is concluded that this inhibin antiserum method is a new practical alternative for induction of superovulation in mice instead of the more commonly used eCG-hCG protocol.
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DOI：
10.1530/jrf.0.0760609
发表时间：
1986
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Quirk,SM;Fortune,JE
通讯作者：
Fortune,JE
影响因子：
3.6
作者：
J. Spearow;P. Nutson;W. Mailliard;M. Porter;M. Barkley
通讯作者：
J. Spearow;P. Nutson;W. Mailliard;M. Porter;M. Barkley
储存孕马血清促性腺激素和人绒毛膜促性腺激素对小鼠诱导排卵的功效。
DOI：
--
发表时间：
1993
期刊：
Laboratory animal science
影响因子：
--
作者：
Garcia,J;Kholkute,SD;Dukelow,WR
通讯作者：
Dukelow,WR
影响因子：
3.6
作者：
SUMMERS, MC;BHATNAGAR, PR;BIGGERS, JD
通讯作者：
BIGGERS, JD