m6A-Related lncRNA to Develop Prognostic Signature and Predict the Immune Landscape in Bladder Cancer.
m6A-Related lncRNA to Develop Prognostic Signature and Predict the Immune Landscape in Bladder Cancer.
复制标题
DOI：
10.1155/2021/7488188
复制
发表时间：
2021
影响因子：
--
通讯作者：
Huang P
中科院分区：
文献类型：
--
作者：
Li Z;Li Y;Zhong W;Huang P
异常m6 A甲基化在癌症进展中起重要作用。越来越多的研究人员专注于开发lncRNA标签来评估癌症患者的预后。m6 A相关lncRNA在膀胱癌患者预后和膀胱癌免疫微环境中的特异性功能仍然是难以捉摸的。在此，我们使用TCGA数据集对m6 A相关lncRNA预后值及其与膀胱癌免疫微环境的相关性进行了全面分析。共有9种m6 A相关lncRNA与膀胱癌的总体生存结局显著相关。通过对9种m6 A相关的预后lncRNA的一致聚类鉴定了两种分子亚型（簇1和簇2）。聚类1与预后差、临床分期晚、PD-L1表达高、ESTIMATEScore和immuneScore高、免疫细胞浸润明显相关。GSEA揭示了在簇2中细胞凋亡和JAK-STAT信号通路的富集。使用9个m6 A相关的预后lncRNA构建预后风险评分，其作为膀胱癌的独立预后因素。此外，低风险评分组的膀胱癌患者具有较高的pN分期、pT分期和临床分期，以及较低的肿瘤分级和免疫评分。风险评分与某些免疫细胞的浸润水平相关，包括B细胞、浆细胞、滤泡辅助T细胞、调节性T细胞、静息NK细胞、中性粒细胞、M0巨噬细胞、M1巨噬细胞和M2巨噬细胞。总的来说，我们的研究阐明了m6 A相关lncRNA在膀胱癌患者预后和膀胱癌免疫微环境中的重要作用。结果表明，m6 A相关的预后lncRNA签名的组分可能作为膀胱癌中免疫微环境的关键介质，代表了改善免疫功效的有希望的治疗靶点。
Abnormal m6A methylation plays a significant role in cancer progression. Increasingly, researchers have focused on developing lncRNA signatures to evaluate the prognosis of cancer patients. The specific function of m6A-related lncRNAs in the prognosis of bladder cancer patients and the immune microenvironment of bladder cancer remains elusive. Herein, we performed a comprehensive analysis of m6A-related lncRNA prognostic values and their association with the immune microenvironment in bladder cancer using the TCGA dataset. A total of 9 m6A-related lncRNAs were dramatically correlated with overall survival outcomes in bladder cancer. Two molecular subtypes (cluster 1 and cluster 2) were identified by consensus clustering for 9 m6A-related prognostic lncRNAs. Cluster 1 was significantly correlated with poor prognosis, advanced clinical stage, higher PD-L1 expression, a higher ESTIMATEScore and immuneScore, and distinct immune cell infiltration. GSEA revealed the enrichment of apoptosis and the JAK-STAT signaling pathway in cluster 2. A prognostic risk score was constructed using 9 m6A-related prognostic lncRNAs, which functioned as an independent prognostic factor for bladder cancer. Moreover, bladder cancer patients in the low-risk score group had a higher pN stage, pT stage, and clinical stage and a lower tumor grade and immuneScore. The risk score was correlated with the infiltration levels of certain immune cells, including B cells, plasma cells, follicular helper T cells, regulatory T cells, resting NK cells, neutrophils, M0 macrophages, M1 macrophages, and M2 macrophages. Collectively, our study elucidated the important role of m6A-related lncRNAs in the prognosis of bladder cancer patients and in the bladder cancer immune microenvironment. The results suggest that the components of the m6A-related prognostic lncRNA signature might serve as a crucial mediator of the immune microenvironment in bladder cancer, representing promising therapeutic targets for improving immunotherapeutic efficacy.
登录
查看更多内容
N6-甲基腺苷相关 lncRNA 是预测低级别胶质瘤患者总体生存率的潜在生物标志物
影响因子：
5.5
作者：
Tu, Zewei;Wu, Lei;Zhu, Xingen
通讯作者：
Zhu, Xingen
影响因子：
3.1
作者：
Liu, Tao;Li, Chenyao;Wang, Lei
通讯作者：
Wang, Lei
影响因子：
8.8
作者：
Yi, Lilan;Wu, Guowu;Huang, Ping
通讯作者：
Huang, Ping
影响因子：
11.2
作者：
Bhan A;Soleimani M;Mandal SS
通讯作者：
Mandal SS
影响因子：
3.6
作者：
Wu, Zonglong;Zhu, Kejia;Shi, Benkang
通讯作者：
Shi, Benkang