Delivery of size-controlled long-circulating polymersomes in solid tumours, visualized by quantum dots and optical imaging in vivo.
Delivery of size-controlled long-circulating polymersomes in solid tumours, visualized by quantum dots and optical imaging in vivo.
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DOI：
10.1080/13102818.2014.984894
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发表时间：
2015-01-02
期刊：
影响因子：
--
通讯作者：
Aoki I
中科院分区：
文献类型：
--
作者：
Bakalova R;Lazarova D;Nikolova B;Atanasova S;Zlateva G;Zhelev Z;Aoki I
本研究旨在研究基于化学修饰壳聚糖的多离子复合中空囊泡(多聚体)是否适合作为药物载体应用于被动肿瘤靶向。这些实验是在结肠癌移植小鼠身上进行的。小鼠麻醉后静脉注射水溶性纳米颗粒：(1)QD705标记的聚合体(平均大小为∼120 nm；大小分布∼为10%)或(2)天然QD705。光学成像在Vivo成像系统中的Maestro EX 2.10上进行(激发滤光片435~480 nm；发射滤光片700 nm，长通)。QD705注射后1分钟内肿瘤区域出现荧光，60分钟内荧光完全消失。第30分钟在肝脏中检测到强荧光信号。使用QD705对肿瘤的可视化仅基于血管生成。在QD705标记的聚合体的情况下，注射后立即在肿瘤区域出现荧光，对全身血管的显示效果很好。16小时内在肿瘤区域检测到强烈的荧光信号。这表明QD705标记的聚合体由于其在血液中的长循环和增强的通透性和滞留效应而主要被输送到肿瘤中。在肝脏区域发现了很弱的荧光信号。这些数据表明，大小可控的长循环多聚体是非常有前景的固体肿瘤药物输送载体，包括小纳米颗粒和对比剂的输送。
The present study was designed to investigate whether poly-ion complex hollow vesicles (polymersomes), based on chemically modified chitosan, are appropriate for passive tumour targeting in the context of their application as drug carriers. The experiments were performed on colon cancer-grafted mice. The mice were subjected to anaesthesia and injected intravenously with water-soluble nanoparticles: (1) QD705-labelled polymersomes (average size ∼120 nm; size distribution ∼10%) or (2) native QD705. The optical imaging was carried out on Maestro EX 2.10 In Vivo Imaging System (excitation filter 435–480 nm; emission filter 700 nm, longpass). In the case of QD705, the fluorescence appeared in the tumour area within 1 min after injection and disappeared completely within 60 min. A strong fluorescent signal was detected in the liver on the 30th minute. The visualization of tumour using QD705 was based only on angiogenesis. In the case of QD705-labelled polymersomes, the fluorescence appeared in the tumour area immediately after injection with excellent visualization of blood vessels in the whole body. A strong fluorescent signal was detected in the tumour area within 16 hours. This indicated that QD705-labelled polymersomes were delivered predominantly into the tumour due to their long circulation in the bloodstream and enhanced permeability and retention effect. A very weak fluorescent signal was found in the liver area. The data suggest that size-controlled long-circulating polymersomes are very promising carriers for drug delivery in solid tumours, including delivery of small nanoparticles and contrast substances.
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影响因子：
8
作者：
Bakalova R;Zhelev Z;Kokuryo D;Spasov L;Aoki I;Saga T
通讯作者：
Saga T
DOI：
10.2217/nnm.10.117
发表时间：
2010-11
期刊：
Nanomedicine (London, England)
影响因子：
--
作者：
Bei D;Meng J;Youan BB
通讯作者：
Youan BB
DOI：
10.1016/j.jconrel.2013.09.013
发表时间：
2013-12-28
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Ernsting MJ;Murakami M;Roy A;Li SD
通讯作者：
Li SD
影响因子：
--
作者：
Muthiah, Muthunarayanan;Lee, Sang Joon;Park, In-Kyu
通讯作者：
Park, In-Kyu
影响因子：
17.1
作者：
Meng H;Xue M;Xia T;Ji Z;Tarn DY;Zink JI;Nel AE
通讯作者：
Nel AE