Neutrophil activation and arteritis induced by C. albicans water-soluble mannoprotein-beta-glucan complex (CAWS).
Neutrophil activation and arteritis induced by C. albicans water-soluble mannoprotein-beta-glucan complex (CAWS).
复制标题
DOI：
10.1016/j.yexmp.2006.05.006
复制
发表时间：
2007-04
影响因子：
3.6
通讯作者：
Suzuki K
中科院分区：
文献类型：
--
作者：
Ishida-Okawara A;Nagi-Miura N;Oharaseki T;Takahashi K;Okumura A;Tachikawa H;Kashiwamura S;Okamura H;Ohno N;Okada H;Ward PA;Suzuki K
我们已经建立了一个小鼠模型，在连续注射CAWS后显示出冠状动脉炎的症状，CAWS是从白色念珠菌释放的。在这项研究中，我们研究了中性粒细胞激活后的初始阶段CAWS腹腔注射。在注射后10分钟至16小时期间，测定血液特征和中性粒细胞功能。同时，测量血浆中炎症细胞因子和趋化因子的水平。此外，测量作为动脉内皮细胞中的损伤标志物的ICAM-1的水平。注射CAWS后，外周血白细胞计数立即升高，尤其是中性粒细胞。体外中性粒细胞对刺激物的敏感性增强。此外，血浆中的促炎细胞因子（IL-1 β、IL-12和IL-6）最初增加，随后IL-10、G-CSF、MIP-2和可溶性ICAM-1增加。局部，ICAM-1在动脉壁的消息显着增加CAWS注射后16小时。在血浆中观察到C3水平降低，表明补体激活和消耗。总之，注射CAWS后发生中性粒细胞活化，随后发生补体活化，并产生促炎细胞因子、趋化因子和G-CSF，这可能参与冠状动脉炎的发生。
We have established a mouse model which shows the symptoms of coronary arteritis after consecutive injections of CAWS, which is released from Candida albicans. In this study, we examined neutrophil activation in the initial period after CAWS injection intraperitoneally. During 10 min to 16 h after the injection, blood profiles and neutrophil functions were determined. At the same time, levels of inflammatory cytokines and chemokines in plasma were measured. Furthermore, level of ICAM-1 as a marker of lesion in arterial endothelial cells was measured. Counts of the peripheral leukocytes increased immediately after CAWS injection, especially involving neutrophil. In vitro sensitivity of neutrophils to stimuli was enhanced. Moreover, proinflammatory cytokines (IL-1β, IL-12 and IL-6) increased in plasma initially followed by an increase in IL-10, G-CSF, MIP-2 and soluble ICAM-1. Locally, ICAM-1 message in arterial walls was significantly increased 16 h after CAWS injection. A decrease in C3 levels was observed in plasma, suggesting complement activation and consumption. In summary, neutrophil activation occurred after CAWS injection, followed by complement activation, and production of proinflammatory cytokines chemokines and G-CSF which may be involved in development of coronary arteritis.
登录
查看更多内容
影响因子：
--
作者：
Gasparoto, TH;Gaziri, LCJ;Felipe, I
通讯作者：
Felipe, I
影响因子：
3.3
作者：
Nagi-Miura, N;Shingo, Y;Ohno, N
通讯作者：
Ohno, N
影响因子：
6.4
作者：
Netea, MG;Stuyt, RJL;Dinarello, CA
通讯作者：
Dinarello, CA
影响因子：
5.5
作者：
Spellberg, BJ;Collins, M;Ibrahim, AS
通讯作者：
Ibrahim, AS
DOI：
10.1084/jem.191.1.189
发表时间：
2000-01-03
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Collins RG;Velji R;Guevara NV;Hicks MJ;Chan L;Beaudet AL
通讯作者：
Beaudet AL