Molecular Characterization and Expression Analysis of the Na(+)/H(+) Exchanger Gene Family in Capsicum annuum L.
Molecular Characterization and Expression Analysis of the Na(+)/H(+) Exchanger Gene Family in Capsicum annuum L.
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辣椒Na/H交换基因家族的分子特征及表达分析。
DOI：
10.3389/fgene.2021.680457
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发表时间：
2021
影响因子：
3.7
通讯作者：
Qin C
中科院分区：
文献类型：
--
作者：
Luo X;Yang S;Luo Y;Qiu H;Li T;Li J;Chen X;Zheng X;Chen Y;Zhang J;Zhang Z;Qin C
Na+/H+ 交换器 (NHX) 是一类参与植物生长和非生物胁迫期间离子平衡的转运蛋白。我们对辣椒CaNHX基因进行了系统的生物信息学鉴定和表达特性分析，为辣椒育种和实际生产提供理论依据。在全基因组水平上，利用生物信息学方法系统鉴定了栽培辣椒和野生辣椒的CaNHX基因家族成员。使用MEGA X软件进行序列比对和系统发育树构建，并对基因功能域、保守基序和基因结构进行分析和可视化。同时对CaNHX基因的共表达网络进行了分析，并对Zunla-1品种在胁迫条件下进行了盐胁迫分析和荧光定量验证。共鉴定出9个CaNHX基因，它们具有Na+/H+交换基因的典型功能域。蛋白质理化性质表明，该蛋白质具有亲水性，氨基酸长度为503~1146个。基因结构分析表明 Chr08 是最定位的染色体，有 8-24 个外显子。顺式作用元件分析表明，其主要含有光反应、水杨酸反应、防御、应激反应等顺式作用元件。转录组和共表达网络分析表明，胁迫下，根和叶中CaNHX基因的共表达基因比对照组更明显，包括ABA、IAA和盐。通过 qRT-PCR 验证转录组和共表达。本研究在辣椒基因组水平上鉴定了CaNHX基因，为提高辣椒的抗逆性、生产、开发利用和遗传育种提供了理论基础。
The Na+/H+ exchangers (NHXs) are a class of transporters involved in ion balance during plant growth and abiotic stress. We performed systematic bioinformatic identification and expression-characteristic analysis of CaNHX genes in pepper to provide a theoretical basis for pepper breeding and practical production. At the whole-genome level, the members of the CaNHX gene family of cultivated and wild pepper were systematically identified using bioinformatics methods. Sequence alignment and phylogenetic tree construction were performed using MEGA X software, and the gene functional domain, conserved motif, and gene structure were analyzed and visualized. At the same time, the co-expression network of CaNHX genes was analyzed, and salt-stress analysis and fluorescence quantitative verification of the Zunla-1 cultivar under stress conditions were performed. A total of 9 CaNHX genes were identified, which have typical functional domains of the Na+/H+ exchanger gene. The physical and chemical properties of the protein showed that the protein was hydrophilic, with a size of 503–1146 amino acids. Analysis of the gene structure showed that Chr08 was the most localized chromosome, with 8–24 exons. Cis-acting element analysis showed that it mainly contains cis-acting elements such as light response, salicylic acid response, defense, and stress response. Transcriptom and co-expression network analysis showed that under stress, the co-expressed genes of CaNHX genes in roots and leaves were more obvious than those in the control group, including ABA, IAA, and salt. The transcriptome and co-expression were verified by qRT-PCR. In this study, the CaNHX genes were identified at the genome level of pepper, which provides a theoretical foundation for improving the stress resistance, production, development, and utilization of pepper in genetic breeding.
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