Hematopoietic growth factors released by marrow stromal cells from patients with aplastic anemia.
Hematopoietic growth factors released by marrow stromal cells from patients with aplastic anemia.
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再生障碍性贫血患者的骨髓基质细胞释放的造血生长因子。
DOI：
10.1182/blood.v79.9.2256.bloodjournal7992256
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发表时间：
1992
期刊：
影响因子：
20.3
通讯作者：
Y. Kodera
中科院分区：
文献类型：
--
作者：
S. Kojima;T. Matsuyama;Y. Kodera
我们研究了33例再生障碍性贫血（AA）患者骨髓基质细胞产生粒细胞集落刺激因子（G-CSF）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和白细胞介素-6（IL-6）的情况。使用长期骨髓培养（LTBMC）系统，33个样本中有29个产生了完整的融合基质层。通过酶联免疫吸附试验（ELISA）测定有或无白细胞介素-1（IL-1）刺激的培养基中G-CSF、GM-CSF和IL-6的浓度。正常对照组和AA患者自发产生的G-CSF、GM-CSF和IL-6无显著差异。基质细胞释放三种造血生长因子对IL-1反应的能力在除一名患者外的所有患者中均正常或升高。我们还研究了16例AA患者抗淋巴细胞球蛋白（ALG）治疗前后基质细胞产生G-CSF、GM-CSF和IL-6的变化。这些细胞因子的产生变化与对ALG的反应之间没有相关性。与先前的研究显示AA患者的基质细胞产生造血生长因子的缺陷相反，结果表明AA患者的骨髓基质细胞产生正常或升高的G-CSF、GM-CSF和IL-6。
We studied the production of granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), and interleukin-6 (IL-6) by stromal cells from 33 patients with aplastic anemia (AA). Complete, confluent stromal layers were produced by 29 of the 33 samples using the long-term bone marrow culture (LTBMC) system. The concentration of G-CSF, GM-CSF, and IL-6 in culture media with or without interleukin-1 (IL-1) stimulation was determined by an enzyme-linked immunoadsorbent assay (ELISA). The spontaneous production of G-CSF, GM-CSF, and IL-6 did not differ significantly between normal controls and the patients with AA. The ability of stromal cells to release the three hematopoietic growth factors in response to IL-1 was either normal or elevated in all but one patient. We also studied the change in production of G-CSF, GM-CSF, and IL-6 by stromal cells before and after antilymphocyte globulin (ALG) therapy in 16 patients with AA. There was no correlation between the change in production of these cytokines and the response to ALG. In contrast to previous studies that showed a defect in the production of hematopoietic growth factors by stromal cells from patients with AA, the results indicated a normal or elevated production of G-CSF, GM-CSF, and IL-6 by marrow stromal cells in patients with AA.
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再生障碍性贫血中功能异常的骨髓基质细胞。
影响因子：
2.6
作者：
Juneja,HS;Gardner,FH
通讯作者：
Gardner,FH
影响因子：
20.3
作者：
G. Sing;J. Keller;L. Ellingsworth;F. Ruscetti
通讯作者：
G. Sing;J. Keller;L. Ellingsworth;F. Ruscetti
DOI：
10.1073/pnas.84.24.9035
发表时间：
1987-12-01
影响因子：
11.1
作者：
IKEBUCHI, K;WONG, GG;OGAWA, M
通讯作者：
OGAWA, M
DOI：
10.1073/pnas.84.17.6020
发表时间：
1987-09-01
影响因子：
11.1
作者：
ASSOIAN, RK;FLEURDELYS, BE;SPORN, MB
通讯作者：
SPORN, MB
影响因子：
15.9
作者：
KAUSHANSKY, K;LIN, N;ADAMSON, JW
通讯作者：
ADAMSON, JW