The novel Pseudomonas aeruginosa two-component regulator BfmR controls bacteriophage-mediated lysis and DNA release during biofilm development through PhdA.
The novel Pseudomonas aeruginosa two-component regulator BfmR controls bacteriophage-mediated lysis and DNA release during biofilm development through PhdA.
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DOI：
10.1111/j.1365-2958.2011.07733.x
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发表时间：
2011-08
影响因子：
3.6
通讯作者：
Sauer K
中科院分区：
文献类型：
--
作者：
Petrova OE;Schurr JR;Schurr MJ;Sauer K
Biofilms are surface-adhered bacterial communities encased in an extracellular matrix composed of polysaccharides, proteins, and extracelluar (e)DNA, with eDNA required for biofilm formation and integrity. Here we demonstrate that eDNA release is controlled by BfmR, a regulator essential for Pseudomonas aeruginosa biofilm development. Expression of bfmR coincided with localized cell death and DNA release, and could be stimulated by conditions resulting in membrane perturbation and cell lysis. ΔbfmR mutant biofilms demonstrated increased cell lysis and eDNA release suggesting BfmR to suppress, but not eliminate, these processes. Genome-wide transcriptional profiling indicated that BfmR was required for repression of genes associated with bacteriophage assembly and bacteriophage-mediated lysis. Chromatin immunoprecipitation analysis of direct BfmR targets identified the promoter of PA0691, termed here phdA, encoding a previously undescribed homologue of the prevent-host-death (Phd) family of proteins. Lack of phdA expression coincided with impaired biofilm development and increased cell death, a phenotype comparable to ΔbfmR. Expression of phdA in ΔbfmR restored eDNA release, cell lysis, and biofilm formation to wild type levels, with phdA overexpression promoting resistance to the superinfective bacteriophage Pf4, detected only in biofilms. Therefore, we propose that BfmR regulates biofilm development by limiting bacteriophage-mediated lysis and thus, eDNA release, via PhdA.
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影响因子：
3.2
作者：
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通讯作者：
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影响因子：
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作者：
Chandrachud U;Gal S
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影响因子：
3.2
作者：
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影响因子：
3.7
作者：
Garcia-Contreras, Rodolfo;Zhang, Xue-Song;Kim, Younghoon;Wood, Thomas K.
通讯作者：
Wood, Thomas K.
影响因子：
5.1
作者：
Barken, Kim B.;Pamp, Suenje J.;Tolker-Nielsen, Tim
通讯作者：
Tolker-Nielsen, Tim