Superantigenic staphylococcal exotoxins induce T-cell proliferation in the presence of Langerhans cells or class II-bearing keratinocytes and stimulate keratinocytes to produce T-cell-activating cytokines.
Superantigenic staphylococcal exotoxins induce T-cell proliferation in the presence of Langerhans cells or class II-bearing keratinocytes and stimulate keratinocytes to produce T-cell-activating cytokines.
复制标题
超抗原葡萄球菌外毒素在朗格汉斯细胞或带有 II 类的角质形成细胞存在的情况下诱导 T 细胞增殖，并刺激角质形成细胞产生 T 细胞激活细胞因子。
DOI：
10.1111/1523-1747.ep12371727
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Tigelaar,RE
中科院分区：
文献类型：
--
作者：
Tokura,Y;Yagi,J;O'Malley,M;Lewis,JM;Takigawa,M;Edelson,RL;Tigelaar,RE
几种葡萄球菌毒素是越来越多的被称为“超级抗原”的免疫刺激分子之一，因为它们在由适当的主要组织相容性复合物 II+ 类辅助细胞呈递时能够激活基本上所有带有特定 T 细胞受体 Vβ 基因片段的 T 细胞。我们检查了鼠表皮朗格汉斯细胞和/或角质形成细胞在 T 细胞对超级抗原葡萄球菌肠毒素 B 和剥脱性毒素（也称为表皮溶血素）的反应中充当辅助细胞的能力。在朗格汉斯细胞（来自正常小鼠的富集表皮细胞或从用重组干扰素-γ预处理的小鼠中分离的表皮细胞）存在下，用葡萄球菌肠毒素 B 或剥脱性毒素刺激纯化的小鼠脾 T 细胞，该过程诱导角质形成细胞上主要组织相容性复合物 II 类分子的表达。数据显示，朗格汉斯细胞和 II 类角质形成细胞均可在 T 细胞对葡萄球菌肠毒素 B 和剥脱性毒素的反应中充当辅助细胞。我们还观察到，在葡萄球菌肠毒素 B 或剥脱性毒素存在下培养的人和鼠角质形成细胞都会产生更多量的能够刺激胸腺细胞和 D10 细胞的细胞因子，并且这种毒素活性与角质形成细胞上 II 类的表达水平无关。酶联免疫吸附试验的研究表明，葡萄球菌肠毒素 B 刺激角质形成细胞产生肿瘤坏死因子-α，但不产生白细胞介素-1，表明肿瘤坏死因子-α 和可能其他细胞因子负责 T 细胞增殖活性。这些结果表明，两种不同的表皮成分（即朗格汉斯细胞和角质形成细胞）可以作为 T 细胞对超抗原细菌毒素的反应中的辅助细胞。这些毒素可能通过局部激活带有特定Vβ基因的T细胞和/或增强角质形成细胞产生免疫调节细胞因子而促进多种皮肤病的发病机制。
Several staphylococcal toxins are among a growing number of immunostimulatory molecules called “superantigens” because of their ability, when presented by appropriate major histocompatibility complex class II+ accessory cells, to activate essentially all T cells bearing particular T-cell receptor Vβ gene segments. We have examined the ability of murine epidermal Langerhans cells and/or keratinocytes to act as accessory cells in the T-cell response to the superantigens staphylococcal enterotoxin B and exfoliative toxin, also known as epidermolysin. Purified murine splenic T cells were stimulated with staphylococcal enterotoxin B or exfoliative toxin in the presence of Langerhans cells–enriched epidermal cells from normal mice or epidermal cells isolated from mice pretreated with recombinant interferon-γ, a procedure that induces the expression of major histocompatibility complex class II molecules on keratinocytes. The data show that both Langerhans cells and class II-bearing keratinocytes can act as accessory cells in the T-cell response to staphylococaal enterotoxin B and exfoliative toxin. We also observed that both human and murine keratinocytes cultured in the presence of staphylococcal enterotoxin B or exfoliative toxin produce increased amounts of cytokine(s) capable of stimulating thymocytes and D10 cells, and that this toxin activity is independent of the level of expression of class II on keratinocytes. Studies by enzyme-linked immunosorbent assay showed that staphylococcal enterotoxin B stimulates keratinocytes to produce tumor necrosis factor-α but not interleukin-1, suggesting tumor necrosis factor-α and perhaps other cytokines are responsible for the T-cell proliferative activity. These results demonstrate that two distinct epidermal constituents (i.e. Langerhans cells and keratinocytes) can serve as accessory cells in the responses of T cells to superantigenic bacterial toxins. It is possible that such toxins contribute to the pathogenesis of a variety of skin diseases by either locally activating T cells bearing particular Vβ genes and/or enhancing keratinocyte production of immunomodulatory cytokines.
登录
查看更多内容
纯化小鼠白细胞介素 1，使其表现出同质性。
影响因子：
4.4
作者：
S. Mizel;D. Mizel
通讯作者：
D. Mizel
对产生毒素 1 的金黄色葡萄球菌的中毒性休克综合征的非化脓性反应。
DOI：
--
发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Fast,DJ;Schlievert,PM;Nelson,RD
通讯作者：
Nelson,RD
人类角质形成细胞在疾病中生物合成 HLA-DR 抗原的证据。
DOI：
10.1084/jem.159.6.1784
发表时间：
1984-06-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Volc-Platzer B;Majdic O;Knapp W;Wolff K;Hinterberger W;Lechner K;Stingl G
通讯作者：
Stingl G
新鲜和培养的朗格汉斯细胞激活半抗原特异性 T 细胞的能力存在差异。
DOI：
--
发表时间：
1993
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Dai,R;Grammer,SF;Streilein,JW
通讯作者：
Streilein,JW
免疫球蛋白超家族的病毒受体
影响因子：
64.5
作者：
J. White;D. Littman
通讯作者：
D. Littman