Characterization of Discrete Phosphopantetheinyl Transferases in Streptomyces tsukubaensis L19 Unveils a Complicate Phosphopantetheinylation Network.
Characterization of Discrete Phosphopantetheinyl Transferases in Streptomyces tsukubaensis L19 Unveils a Complicate Phosphopantetheinylation Network.
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DOI：
10.1038/srep24255
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发表时间：
2016-04-07
影响因子：
4.6
通讯作者：
Li YQ
中科院分区：
文献类型：
--
作者：
Wang YY;Zhang XS;Luo HD;Ren NN;Jiang XH;Jiang H;Li YQ
磷酸泛酰巯基乙胺基转移酶（PPTases）通过酰基载体蛋白（ACP）和肽基载体蛋白（PCP）的翻译后修饰在初级代谢和次级代谢中起重要作用。在这项研究中，FK 506的工业生产菌株Streptomyces tsukubaensis L19，连同Streptomyces avermiatum，被鉴定为含有最高数量（5）的离散PPT酶已知的任何物种中迄今为止检查。对S. tsukubaensisL 19揭示了Ⅱ型PKS中的ACP被三种PPT酶FKPPT 1、FKPPT 3和FKACPS磷酸泛酰巯基乙胺化，脂肪酸合成酶（FAS）中的ACP被三种PPT酶FKPPT 2、FKPPT 3和FKACPS磷酸泛酰巯基乙胺化，FK 506生物合成中的ACP被两种PPT酶FKPPT 3和FKACPS磷酸泛酰巯基乙胺化，TcsA-ACP被两种PPT酶FKPPT 3和FKACPS磷酸泛酰巯基乙胺化，TcsA-ACP被两种PPT酶FKPPT 3和FKACPS磷酸泛酰巯基乙胺化。FkbP-PCP是一种参与FK 506生物合成的PCP，被所有这五种PPT酶FKPPT 1 -4和FKACPS磷酸泛酰巯基乙胺化。我们的研究结果表明，这些PPT酶对于ACP/PCP底物的功能相互补充，表明S中存在复杂的磷酸泛酰巯基乙胺化网络。tsukubaensis L19.在S. tsukubaensis L19产生了一个突变菌株，可以提高FK 506的生产。
Phosphopantetheinyl transferases (PPTases) play essential roles in both primary metabolisms and secondary metabolisms via post-translational modification of acyl carrier proteins (ACPs) and peptidyl carrier proteins (PCPs). In this study, an industrial FK506 producing strain Streptomyces tsukubaensis L19, together with Streptomyces avermitilis, was identified to contain the highest number (five) of discrete PPTases known among any species thus far examined. Characterization of the five PPTases in S. tsukubaensis L19 unveiled that stw ACP, an ACP in a type II PKS, was phosphopantetheinylated by three PPTases FKPPT1, FKPPT3, and FKACPS; sts FAS ACP, the ACP in fatty acid synthase (FAS), was phosphopantetheinylated by three PPTases FKPPT2, FKPPT3, and FKACPS; TcsA-ACP, an ACP involved in FK506 biosynthesis, was phosphopantetheinylated by two PPTases FKPPT3 and FKACPS; FkbP-PCP, an PCP involved in FK506 biosynthesis, was phosphopantetheinylated by all of these five PPTases FKPPT1-4 and FKACPS. Our results here indicate that the functions of these PPTases complement each other for ACPs/PCPs substrates, suggesting a complicate phosphopantetheinylation network in S. tsukubaensis L19. Engineering of these PPTases in S. tsukubaensis L19 resulted in a mutant strain that can improve FK506 production.
II 组磷酸泛酰基转移酶中三联体 Asp-Xxx-Glu 的表征和进化意义。
影响因子：
3.7
作者：
Wang YY;Li YD;Liu JB;Ran XX;Guo YY;Ren NN;Chen X;Jiang H;Li YQ
通讯作者：
Li YQ