Characterization and evolutionary implications of the triad Asp-Xxx-Glu in group II phosphopantetheinyl transferases.
Characterization and evolutionary implications of the triad Asp-Xxx-Glu in group II phosphopantetheinyl transferases.
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II 组磷酸泛酰基转移酶中三联体 Asp-Xxx-Glu 的表征和进化意义。
DOI：
10.1371/journal.pone.0103031
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Li YQ
中科院分区：
文献类型：
--
作者：
Wang YY;Li YD;Liu JB;Ran XX;Guo YY;Ren NN;Chen X;Jiang H;Li YQ
磷酸antetheinyl transferases （PPTases）是镁结合酶，在初级和次级代谢中起重要作用。在这项研究中，我们通过生化和进化分析对所有已知的II族PPTases的镁结合残基进行了表征。结果表明，II类PPTases可分为两个亚群，即含有三联体Asp-Xxx-Glu的2 -镁结合残基PPTases和含有三联体Asp-Glu-Glu的3 -镁结合残基PPTases。两个3 -镁结合残基- pptase和一个2 -镁结合残基- pptase的突变表明，三联体中的第1和第3个残基对活性至关重要；三联式中的第二个残基是不必要的。虽然Asp-Xxx-Glu三位一体中的第二残基在整个系统发育树中都存在变异，但第二残基分别在动物、植物、藻类和大多数原核生物中是保守的。进化分析表明：动物II类PPTases可能起源于一个共同的祖先；植物二镁结合残基pptases可能来自同一个祖先；植物三镁结合残基pptases可能来源于原核生物的水平基因转移。
Phosphopantetheinyl transferases (PPTases), which play an essential role in both primary and secondary metabolism, are magnesium binding enzymes. In this study, we characterized the magnesium binding residues of all known group II PPTases by biochemical and evolutionary analysis. Our results suggested that group II PPTases could be classified into two subgroups, two-magnesium-binding-residue-PPTases containing the triad Asp-Xxx-Glu and three-magnesium-binding-residue-PPTases containing the triad Asp-Glu-Glu. Mutations of two three-magnesium-binding-residue-PPTases and one two-magnesium-binding-residue-PPTase indicate that the first and the third residues in the triads are essential to activities; the second residues in the triads are non-essential. Although variations of the second residues in the triad Asp-Xxx-Glu exist throughout the whole phylogenetic tree, the second residues are conserved in animals, plants, algae, and most prokaryotes, respectively. Evolutionary analysis suggests that: the animal group II PPTases may originate from one common ancestor; the plant two-magnesium-binding-residue-PPTases may originate from one common ancestor; the plant three-magnesium-binding-residue-PPTases may derive from horizontal gene transfer from prokaryotes.
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