Instillation versus inhalation of multiwalled carbon nanotubes: exposure-related health effects, clearance, and the role of particle characteristics.
Instillation versus inhalation of multiwalled carbon nanotubes: exposure-related health effects, clearance, and the role of particle characteristics.
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DOI：
10.1021/nn503887r
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发表时间：
2014-09-23
期刊：
影响因子：
17.1
通讯作者：
Pinkerton, Kent E.
中科院分区：
文献类型：
--
作者：
Silva, Rona M.;Doudrick, Kyle;Franzi, Lisa M.;TeeSy, Christel;Anderson, Donald S.;Wu, Zheqiong;Mitra, Somenath;Vu, Vincent;Dutrow, Gavin;Evans, James E.;Westerhoff, Paul;Van Winkle, Laura S.;Raabe, Otto G.;Pinkerton, Kent E.
关键词：
吸入多壁碳纳米管（MWCNTs）可能会导致不良的肺部反应，由于其纳米级，纤维状形态和/或生物持久性。本研究测试了影响MWCNT毒性的多个因素（剂量、时间、理化特性和给药方法），假设这些因素将影响MWCNT暴露后的显著不同反应。该研究的独特之处在于：（1）使用来自相同库存的颗粒测试了多种给药方法;（2）散装MWCNT制剂几乎没有差异（金属含量，表面积/功能化）;（3）使用专门的方法定量测定了MWCNT在啮齿动物肺部的保留。雄性Sprague-Dawley大鼠通过气管内滴注和吸入暴露于原始（0）、纯化（P）和羧酸官能化（F）MWCNT。在给药后第1天和第21天收集血液、支气管肺泡灌洗液（BALF）和肺组织，通过程序热分析定量肺组织中的生物学反应和MWCNT。在第1天，MWCNT滴注产生显著的BALF嗜中性粒细胞和MWCNT阳性巨噬细胞。灌输O-和P-MWCNT在肺组织中产生显著的炎症，尽管MWCNT保留，但到第21天该炎症消退。在第1天后，MWCNT吸入未产生BALF嗜中性粒细胞，也未产生显著的组织病理学。然而，在吸入雾化多壁碳纳米管后的第1天和第21天，在BALF中观察到多壁碳纳米管阳性巨噬细胞的数量显著增加。结果表明：（1）多壁碳纳米管产生短暂的炎症，如果有的话，尽管在肺中的持久性;（2）灌输O-MWCNT引起更多的炎症比P-或F-MWCNT;和（3）MWCNT悬浮液介质产生显着不同的影响，物理化学颗粒特性和肺反应。
Inhaled multiwalled carbon nanotubes (MWCNTs) may cause adverse pulmonary responses due to their nanoscale, fibrous morphology and/or biopersistance. This study tested multiple factors (dose, time, physicochemical characteristics, and administration method) shown to affect MWCNT toxicity with the hypothesis that these factors will influence significantly different responses upon MWCNT exposure. The study is unique in that (1) multiple administration methods were tested using particles from the same stock; (2) bulk MWCNT formulations had few differences (metal content, surface area/functionalization); and (3) MWCNT retention was quantified using a specialized approach for measuring unlabeled MWCNTs in rodent lungs. Male Sprague–Dawley rats were exposed to original (O), purified (P), and carboxylic acid functionalized (F) MWCNTs via intratracheal instillation and inhalation. Blood, bronchoalveolar lavage fluid (BALF), and lung tissues were collected at postexposure days 1 and 21 for quantifying biological responses and MWCNTs in lung tissues by programmed thermal analysis. At day 1, MWCNT instillation produced significant BALF neutrophilia and MWCNT-positive macrophages. Instilled O- and P-MWCNTs produced significant inflammation in lung tissues, which resolved by day 21 despite MWCNT retention. MWCNT inhalation produced no BALF neutrophilia and no significant histopathology past day 1. However, on days 1 and 21 postinhalation of nebulized MWCNTs, significantly increased numbers of MWCNT-positive macrophages were observed in BALF. Results suggest (1) MWCNTs produce transient inflammation if any despite persistence in the lungs; (2) instilled O-MWCNTs cause more inflammation than P- or F-MWCNTs; and (3) MWCNT suspension media produce strikingly different effects on physicochemical particle characteristics and pulmonary responses.
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影响因子：
10.4
作者：
Bonner JC;Silva RM;Taylor AJ;Brown JM;Hilderbrand SC;Castranova V;Porter D;Elder A;Oberdörster G;Harkema JR;Bramble LA;Kavanagh TJ;Botta D;Nel A;Pinkerton KE
通讯作者：
Pinkerton KE
影响因子：
17.1
作者：
Cheng, Jinping;Fernando, K. A. Shiral;Cheng, Shuk Han
通讯作者：
Cheng, Shuk Han
影响因子：
10
作者：
Hamilton RF Jr;Wu Z;Mitra S;Shaw PK;Holian A
通讯作者：
Holian A
影响因子：
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作者：
Chen, Yuhong;Mitra, Somenath
通讯作者：
Mitra, Somenath
影响因子：
4.1
作者：
Jain, Sanyog;Thakare, Vivek S.;Mishra, Anil K.
通讯作者：
Mishra, Anil K.