Anti-Allergic and Anti-Inflammatory Effects of Neferine on RBL-2H3 Cells.
Anti-Allergic and Anti-Inflammatory Effects of Neferine on RBL-2H3 Cells.
复制标题
NEFERINE对RBL-2H3细胞的抗过敏和抗炎作用。
DOI：
10.3390/ijms222010994
复制
发表时间：
2021-10-12
影响因子：
5.6
通讯作者：
Hung CF
中科院分区：
文献类型：
--
作者：
Chiu KM;Hung YL;Wang SJ;Tsai YJ;Wu NL;Liang CW;Chang DC;Hung CF
肥大细胞在皮肤过敏和炎症中起着非常重要的作用，包括特应性皮炎和银屑病。过去，人们发现甲基莲心碱对皮肤具有抗炎和抗衰老作用，但其对肥大细胞的影响尚未进行详细研究。本研究采用肥大细胞（RBL-2 H3细胞）和小鼠模型研究甲基莲心碱的抗过敏和炎症作用。首先，我们发现甲基莲心碱抑制肥大细胞的脱颗粒和细胞因子的表达。此外，我们观察到，当肥大细胞被A23187/佛波醇12-肉豆蔻酸酯-13-乙酸酯（PMA）刺激时，细胞内钙的升高被甲基莲心碱抑制。甲基莲心碱预处理可降低MAPK/NF-κB通路的磷酸化水平。体内研究结果表明甲基莲心碱可改善二硝基氯苯（DNCB）引起的皮炎和肥大细胞浸润。此外，皮肤中屏障蛋白的表达也得到恢复。最后，发现甲基莲心碱可以减少由化合物48/80引起的刮擦行为。综上所述，我们的研究结果表明甲基莲心碱是一种非常好的抗过敏和抗炎的天然产物。其对肥大细胞的影响有助于其药理学机制。
Mast cells play a very important role in skin allergy and inflammation, including atopic dermatitis and psoriasis. In the past, it was found that neferine has anti-inflammatory and anti-aging effects on the skin, but its effect on mast cells has not yet been studied in detail. In this study, we used mast cells (RBL-2H3 cells) and mouse models to study the anti-allergic and inflammatory effects of neferine. First, we found that neferine inhibits the degranulation of mast cells and the expression of cytokines. In addition, we observed that when mast cells were stimulated by A23187/phorbol 12-myristate-13-acetate (PMA), the elevation of intracellular calcium was inhibited by neferine. The phosphorylation of the MAPK/NF-κB pathway is also reduced by pretreatment of neferine. The results of in vivo studies show that neferine can improve the appearance of dermatitis and mast cell infiltration caused by dinitrochlorobenzene (DNCB). Moreover, the expressions of barrier proteins in the skin are also restored. Finally, it was found that neferine can reduce the scratching behavior caused by compound 48/80. Taken together, our results indicate that neferine is a very good anti-allergic and anti-inflammatory natural product. Its effect on mast cells contributes to its pharmacological mechanism.
登录
查看更多内容
DOI：
10.1016/0922-4106(92)90086-b
发表时间：
1992-05-12
期刊：
EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION
影响因子：
--
作者：
KOCH, G;HABERMANN, B;AKTORIES, K
通讯作者：
AKTORIES, K
影响因子：
5
作者：
Kono, Ryohei;Nomura, Sachiko;Ueno, Masami
通讯作者：
Ueno, Masami
DOI：
10.1016/j.jaci.2017.01.011
发表时间：
2017-04
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Brunner PM;Guttman-Yassky E;Leung DY
通讯作者：
Leung DY
影响因子：
64.8
作者：
McNeil, Benjamin D.;Pundir, Priyanka;Meeker, Sonya;Han, Liang;Undem, Bradley J.;Kulka, Marianna;Dong, Xinzhong
通讯作者：
Dong, Xinzhong
影响因子：
5.4
作者：
BARSUMIAN, EL;ISERSKY, C;SIRAGANIAN, RP
通讯作者：
SIRAGANIAN, RP