The Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid Alleviates Salinity Stress in Cassava.
The Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid Alleviates Salinity Stress in Cassava.
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DOI：
10.3389/fpls.2016.02039
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发表时间：
2016
影响因子：
5.6
通讯作者：
Seki M
中科院分区：
文献类型：
--
作者：
Patanun O;Ueda M;Itouga M;Kato Y;Utsumi Y;Matsui A;Tanaka M;Utsumi C;Sakakibara H;Yoshida M;Narangajavana J;Seki M
木薯（Manihot esculenta Crantz）的需求一直在上升，因为它的各种应用。高盐胁迫是干扰植物正常生长和限制作物产量的主要环境因素。除了利用基因工程提高抗逆性外，利用小分子技术也被认为是一种使植物具有所需性状的替代方法。最近报道了组蛋白去乙酰化酶（HDAC）抑制剂对提高盐度胁迫耐受性的有效性。在这里，我们使用HDAC抑制剂亚甲基苯胺羟肟酸（SAHA）来增强木薯对高盐度的耐受性。免疫印迹分析显示，SAHA处理可诱导根中组蛋白H3和H4的强烈超乙酰化，表明SAHA在木薯中起HDAC抑制剂的作用。与SAHA处理提高了对盐胁迫的耐受性一致，SAHA处理的植株Na+含量降低，K+/Na+比值升高。通过转录组分析发现，通过SAHA处理介导的盐胁迫耐受机制显示，SAHA在正常条件下提高了421个基因的表达，在SAHA和NaCl处理下，在2 h和24 h分别提高了745个基因和268个基因的表达。在saha预处理的根中，高盐处理后参与植物激素[脱落酸（ABA）、茉莉酸（JA）、乙烯和赤霉素]生物合成途径的基因mRNA表达上调。其中，在盐胁迫条件下，参与JA生物合成关键步骤的烯氧化物环化酶（MeAOC4）在SAHA处理下被强烈上调，这表明JA途径可能有助于SAHA处理提高JA的耐盐性。我们的研究结果表明，表观遗传操作可能增强木薯对高盐胁迫的耐受性。
Cassava (Manihot esculenta Crantz) demand has been rising because of its various applications. High salinity stress is a major environmental factor that interferes with normal plant growth and limits crop productivity. As well as genetic engineering to enhance stress tolerance, the use of small molecules is considered as an alternative methodology to modify plants with desired traits. The effectiveness of histone deacetylase (HDAC) inhibitors for increasing tolerance to salinity stress has recently been reported. Here we use the HDAC inhibitor, suberoylanilide hydroxamic acid (SAHA), to enhance tolerance to high salinity in cassava. Immunoblotting analysis reveals that SAHA treatment induces strong hyper-acetylation of histones H3 and H4 in roots, suggesting that SAHA functions as the HDAC inhibitor in cassava. Consistent with increased tolerance to salt stress under SAHA treatment, reduced Na+ content and increased K+/Na+ ratio were detected in SAHA-treated plants. Transcriptome analysis to discover mechanisms underlying salinity stress tolerance mediated through SAHA treatment reveals that SAHA enhances the expression of 421 genes in roots under normal condition, and 745 genes at 2 h and 268 genes at 24 h under both SAHA and NaCl treatment. The mRNA expression of genes, involved in phytohormone [abscisic acid (ABA), jasmonic acid (JA), ethylene, and gibberellin] biosynthesis pathways, is up-regulated after high salinity treatment in SAHA-pretreated roots. Among them, an allene oxide cyclase (MeAOC4) involved in a crucial step of JA biosynthesis is strongly up-regulated by SAHA treatment under salinity stress conditions, implying that JA pathway might contribute to increasing salinity tolerance by SAHA treatment. Our results suggest that epigenetic manipulation might enhance tolerance to high salinity stress in cassava.
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影响因子：
4.3
作者：
Kaldis, Athanasios;Tsementzi, Despoina;Vlachonasios, Konstantinos E.
通讯作者：
Vlachonasios, Konstantinos E.
影响因子：
11.6
作者：
Moller, Inge S.;Gilliham, Matthew;Tester, Mark
通讯作者：
Tester, Mark
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
56.9
作者：
Apse, MP;Aharon, GS;Blumwald, E
通讯作者：
Blumwald, E
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F