Comparison of Glutathione, Cysteine, and Their Redox Potentials in the Plasma of Critically Ill and Healthy Children.
Comparison of Glutathione, Cysteine, and Their Redox Potentials in the Plasma of Critically Ill and Healthy Children.
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DOI：
10.3389/fped.2015.00046
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发表时间：
2015
影响因子：
2.6
通讯作者：
Hebbar KB
中科院分区：
文献类型：
--
作者：
Grunwell JR;Gillespie SE;Ward JM;Fitzpatrick AM;Brown LA;Gauthier TW;Hebbar KB
已知氧化应激由于活性氧和活性氮的不平衡以及身体使用小分子抗氧化剂和抗氧化酶来解毒促氧化剂的能力而在危重病中发挥作用。比较危重病儿童与健康对照儿童的血浆氧化还原代谢物浓度和Cys/CySS和GSH/GSSG巯基/二硫键对的氧化还原电位。我们对2010年1月至2012年4月期间在30张床位的多学科内外科儿科重症监护病房（PICU）住院的年龄≤18岁且体重≥6 kg的儿童进行了一项前瞻性临床观察性研究。我们测量了Cys，CySS，GSH和GSSG的血浆浓度在第一个24小时的PICU的到来，我们计算了氧化还原电位的Cys/CySS（Eh Cys/CySS）和GSH/GSSG（Eh GSH/GSSG）巯基/二硫键对在61例危重病儿童和16例健康对照儿童的血浆中。与健康儿童相比，重症儿童具有更少的Cys（p = 0.009）、更少的CySS（p = 0.011）、更少的总Cys（[Cys]+2 [CySS]，p = 0.01）、更多的GSSG（p < 0.001）和更多的氧化型Eh GSH/GSSG（p < 0.001）。我们的研究结果表明，在儿科危重病的存在下，总Cys/CySS巯基池减少，而GSH可能是细胞氧化还原系统的一个组成部分，将CySS还原为Cys，从而维持Eh Cys/CySS。在儿童血浆中，总Cys池比总GSH池更丰富。需要进一步的研究来阐明危重患儿亚组中氧化还原电位的差异，并确定氧化还原代谢物浓度和氧化还原电位的差异是否与危重病的严重程度和临床结局相关。
Oxidative stress is known to play a role in critical illness due to an imbalance in reactive oxygen species and reactive nitrogen species, and the body’s ability to detoxify pro-oxidants using small molecule anti-oxidants and anti-oxidant enzymes. To compare the concentrations of plasma redox metabolites and redox potentials for the Cys/CySS and GSH/GSSG thiol/disulfide pairs in critically ill children with healthy control children. We performed a prospective clinical observational study of children ages ≤18 years and weight ≥6 kg, who were hospitalized between January 2010 and April 2012 in a 30-bed multidisciplinary medical-surgical pediatric intensive care unit (PICU). We measured the plasma concentrations of Cys, CySS, GSH, and GSSG within the first 24 h of PICU arrival, and we calculated the redox potential for the Cys/CySS (Eh Cys/CySS) and GSH/GSSG (Eh GSH/GSSG) thiol/disulfide pairs in the plasma of 61 critically ill children and 16 healthy control children. Critically ill children have less Cys (p = 0.009), less CySS (p = 0.011), less Total Cys ([Cys] + 2[CySS], p = 0.01), more GSSG (p < 0.001), and more oxidized Eh GSH/GSSG (p < 0.001) compared to healthy children. Our results demonstrate that in the presence of pediatric critical illness, the Total Cys/CySS thiol pool decreases while GSH is likely one component of the cellular redox system that reduces CySS back to Cys, thus maintaining Eh Cys/CySS. The Total Cys pool is more abundant than the Total GSH pool in the plasma of children. Further investigation is needed to elucidate the differences in redox potentials in subgroups of critically ill children, and to determine whether differences in redox metabolite concentrations and redox potentials correlate with severity of critical illness and clinical outcomes.
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影响因子：
7.4
作者：
Kadiiska, Maria B.;Peddada, Shyamal;Herbert, Ronald A.;Basu, Samar;Hensley, Kenneth;Jones, Dean P.;Hatch, Gary E.;Mason, Ronald P.
通讯作者：
Mason, Ronald P.
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Bar-Or, David;Bar-Or, Raphael;Rael, Leonard T.;Brody, Edward N.
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作者：
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Sternberg, P
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作者：
Jones, DP;Carlson, JL;Brown, LAS
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