Antioxidative Effects of Cherry Leaves Extract on tert-Butyl Hydroperoxide-Mediated Cytotoxicity Through Regulation of Thioredoxin-2 Protein Expression Levels
Antioxidative Effects of Cherry Leaves Extract on tert-Butyl Hydroperoxide-Mediated Cytotoxicity Through Regulation of Thioredoxin-2 Protein Expression Levels
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樱桃叶提取物通过调节硫氧还蛋白-2 蛋白表达水平对叔丁基过氧化氢介导的细胞毒性的抗氧化作用
DOI：
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Masashi Kato
中科院分区：
文献类型：
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作者：
Nobuhiko Taguchi;Noriyuki Uemura;Yuji Goto;M. Sakura;Kazuhiro Hara;M. Niwa;Machiko Iida;T. Yanagishita;D. Watanabe;Masashi Kato
樱桃树的成分已被用作治疗各种疾病的传统草药。已知这些成分具有抗氧化作用。然而，樱桃树成分介导的抗氧化作用的机制仍然是未知的。本研究以樱桃叶提取物（CLE）为研究对象，探讨了CLE对过氧化叔丁基（t-BOOH）诱导的黑色素细胞死亡伴DNA损伤的作用机制。有趣的是，CLE通过减少DNA损伤、p38激酶激活和活性氧（ROS）产生来阻止t- boo诱导的细胞死亡。硫氧还蛋白（Trx）系统抑制剂可阻止cle介导的细胞死亡抑制，减少DNA损伤、p38激酶活性和ROS产生，而谷胱甘肽（GSH）系统抑制剂则不能。最后，数据显示CLE阻止了t- boo诱导的Trx2的减少，但不阻止Trx1和Trx还原酶（TrxR1和TrxR2）蛋白的表达。因此，我们的研究结果表明，CLE可阻止t- boo诱导的Trx2表达降低、ROS生成促进、p38激酶激活和DNA损伤增加，并可防止细胞死亡。
Components of cherry trees have been used as traditional herbal remedies for various diseases. These components are known to possess antioxidative effects. However, the mechanisms underlying cherry tree component-mediated antioxidative effects remain largely unknown. This study focused on cherry leaves extract (CLE) and examined the mechanism underlying the effect of CLE on tert-butyl hydroperoxide (t-BOOH)-induced melanocytic cell death with DNA damage. Interestingly, CLE prevented t-BOOH-induced cell death with reduction in DNA damage, p38 kinase activation, and reactive oxygen species (ROS) production. CLE-mediated suppression of cell death with reduction of DNA damage, p38 kinase activity and ROS production was prevented by a thioredoxin (Trx) system inhibitor but not by a glutathione (GSH) system inhibitor. Finally, data showed that CLE prevented t-BOOH-induced reduction of Trx2 but not Trx1 and Trx reductases (TrxR1 and TrxR2) protein expression. Thus, our results suggest that CLE prevents t-BOOH-induced reduction in Trx2 expression, promotion of ROS production, activation of p38 kinase, and increase in DNA damage and that it protects against cell death.
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影响因子：
4.4
作者：
J. Cai;M. Wu;K. C. Nelson;P. Sternberg;D. P. Jones
通讯作者：
J. Cai;M. Wu;K. C. Nelson;P. Sternberg;D. P. Jones
影响因子：
4.4
作者：
Maher, P;Hanneken, A
通讯作者：
Hanneken, A
影响因子：
4.4
作者：
Hanneken, Anne;Lin, Fen-Fen;Maher, Pamela
通讯作者：
Maher, Pamela
影响因子：
6.6
作者：
Bindoli A;Fukuto JM;Forman HJ
通讯作者：
Forman HJ
抗氧化剂抑制 LLC-PK1 细胞中碘乙酰胺和叔丁基氢过氧化物的毒性：脂质过氧化在烷基化诱导的细胞毒性中的作用。
影响因子：
3.9
作者：
Chen,Q;Stevens,JL
通讯作者：
Stevens,JL