Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique.
Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique.
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DOI：
10.3390/bios8040097
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发表时间：
2018-10-20
期刊：
影响因子：
--
通讯作者：
Sones CL
中科院分区：
文献类型：
--
作者：
He PJW;Katis IN;Eason RW;Sones CL
纸基横向流动装置(LFD)被认为是理想的低成本诊断解决方案，适用于医疗保健(POC)场景，允许快速检测流体样本中的单个分析物，并已普遍使用了十年。近年来，对单个样品中存在的多个分析物的快速和同时检测的需求越来越大，为了促进这一点，我们在此报告了一种新的解决方案-使用我们之前报道的激光直写(LDW)技术通过精确划分标准LFD的单一流动路径创建的多路径LFD进行检测。多个流动路径允许在每个平行通道内分别同时检测不同的分析物，而没有任何交叉反应。个别通道中彩色测试线的出现表明样品中存在不同的分析物。我们成功地提出了使用LDW模式的多路径LFD对包括C-反应蛋白(CRP)和血清淀粉样蛋白A-1(SAA1)的生物标志物面板进行多重检测，用于诊断细菌感染。总体而言，我们展示了在创建新的LFD中使用我们的LDW技术，该技术能够对单个LFD内的两个炎症标志物进行多路复用检测，从而提供了一种相对比标准顺序多路复用过程更有效的检测方案。
Paper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. In recent years, there has been an increasing need for rapid and simultaneous detection of multiple analytes present within a single sample and to facilitate this, we report here a novel solution—detection using a multi-path LFD created via the precise partitioning of the single flow-path of a standard LFD using our previously reported laser direct-write (LDW) technique. The multiple flow-paths allow the simultaneous detection of the different analytes individually within each of the parallel channels without any cross-reactivity. The appearance of coloured test lines in individual channels indicates the presence of the different analytes within a sample. We successfully present the use of a LDW-patterned multi-path LFD for multiplexed detection of a biomarker panel comprising C-reactive protein (CRP) and Serum amyloid A-1 (SAA1), used for the diagnosis of bacterial infections. Overall, we demonstrate the use of our LDW technique in the creation of a novel LFD that enables multiplexed detection of two inflammation markers within a single LFD providing a detection protocol that is comparatively more efficient than the standard sequential multiplexing procedure.
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影响因子：
24.5
作者：
Moreno ML;Cebolla Á;Muñoz-Suano A;Carrillo-Carrion C;Comino I;Pizarro Á;León F;Rodríguez-Herrera A;Sousa C
通讯作者：
Sousa C
影响因子：
4.4
作者：
Spiro, A;Lowe, M;Brown, D
通讯作者：
Brown, D
影响因子：
6.4
作者：
Koczula KM;Gallotta A
通讯作者：
Gallotta A
影响因子：
15.9
作者：
Pepys, MB;Hirschfield, GM
通讯作者：
Hirschfield, GM
影响因子：
2.7
作者：
Mendoza, LG;McQuary, P;Eggers, M
通讯作者：
Eggers, M