The reduced genome of the parasitic microsporidian Enterocytozoon bieneusi lacks genes for core carbon metabolism.
The reduced genome of the parasitic microsporidian Enterocytozoon bieneusi lacks genes for core carbon metabolism.
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DOI：
10.1093/gbe/evq022
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发表时间：
2010-07-12
影响因子：
3.3
通讯作者：
Tzipori S
中科院分区：
文献类型：
--
作者：
Keeling PJ;Corradi N;Morrison HG;Haag KL;Ebert D;Weiss LM;Akiyoshi DE;Tzipori S
各种生物过程的减少是寄生生活方式的标志。一般来说，寄生虫与宿主之间的联系越密切，寄生虫对其宿主生理机能的生存和繁殖的依赖就越强。然而，有些系统被认为是不可或缺的，例如从糖类产生能量的碳代谢的核心途径。即使是缺乏氧化磷酸化和三羧酸循环的最顽固的厌氧菌也保留了糖酵解和一些产生 ATP 的下游手段。在这里，我们描述了致病性小孢子虫（Enterocytozoon bieneusi）的深度覆盖基因组重测序，这表明这种寄生虫已经跨越了这条线并放弃了最基本的碳代谢的完整途径。将 E. bieneusi 的两次基因组序列调查与其他四种微孢子虫的基因组数据进行比较，发现代表 E. bieneusi 中 30 个功能途径的 353 个基因的正常互补，除了共同参与糖酵解、磷酸戊糖和海藻糖代谢的 21 个基因中仅存在 2 个。同样，没有发现编码参与剪接体内含子加工的蛋白质的基因。总而言之，E. bieneusi 似乎没有从葡萄糖生成 ATP 的功能齐全的途径。因此，这种细胞内寄生虫依赖转运蛋白从宿主输入 ATP。
Reduction of various biological processes is a hallmark of the parasitic lifestyle. Generally, the more intimate the association between parasites and hosts the stronger the parasite relies on its host's physiology for survival and reproduction. However, some systems have been held to be indispensable, for example, the core pathways of carbon metabolism that produce energy from sugars. Even the most hardened anaerobes that lack oxidative phosphorylation and the tricarboxylic acid cycle have retained glycolysis and some downstream means to generate ATP. Here we describe the deep-coverage genome resequencing of the pathogenic microsporidiian, Enterocytozoon bieneusi, which shows that this parasite has crossed this line and abandoned complete pathways for the most basic carbon metabolism. Comparing two genome sequence surveys of E. bieneusi to genomic data from four other microsporidia reveals a normal complement of 353 genes representing 30 functional pathways in E. bieneusi, except that only 2 out of 21 genes collectively involved in glycolysis, pentose phosphate, and trehalose metabolism are present. Similarly, no genes encoding proteins involved in the processing of spliceosomal introns were found. Altogether, E. bieneusi appears to have no fully functional pathway to generate ATP from glucose. Therefore, this intracellular parasite relies on transporters to import ATP from its host.
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影响因子：
12.3
作者：
Milinkovitch MC;Helaers R;Depiereux E;Tzika AC;Gabaldón T
通讯作者：
Gabaldón T
对不可培养的机会性人类病原体，肠肠细胞生物的基因组调查。
影响因子：
6.7
作者：
Akiyoshi DE;Morrison HG;Lei S;Feng X;Zhang Q;Corradi N;Mayanja H;Tumwine JK;Keeling PJ;Weiss LM;Tzipori S
通讯作者：
Tzipori S
影响因子：
2.5
作者：
Richards, TA;Hirt, RP;Embley, TM
通讯作者：
Embley, TM
影响因子：
64.8
作者：
Margulies, M;Egholm, M;Rothberg, JM
通讯作者：
Rothberg, JM
影响因子：
14.9
作者：
Delcher, AL;Harmon, D;Salzberg, SL
通讯作者：
Salzberg, SL