A 33 kDa protein with sequence homology to the 'laminin binding protein' is associated with the cytoskeleton in hydra and in mammalian cells.
A 33 kDa protein with sequence homology to the 'laminin binding protein' is associated with the cytoskeleton in hydra and in mammalian cells.
复制标题
与“层粘连蛋白结合蛋白”具有序列同源性的 33 kDa 蛋白质与水螅和哺乳动物细胞中的细胞骨架相关。
作者：
E. Keppel;H. Schaller
在水螅和哺乳动物细胞中，单克隆抗体V识别与细胞骨架结构共定位的表位。使用该抗体进行表达筛选，从水螅中分离cDNA克隆（955 bp），其覆盖294个氨基酸的蛋白质的开放阅读框，计算的分子量为32.8 kDa。水螅RNA的北方印迹分析得到1.2 kb的单个mRNA种类，引物延伸实验证明这是全长信息。在水螅蛋白的氨基末端的218个残基显示出与命名为“层粘连蛋白结合蛋白”的人蛋白的广泛同源性（73.5%）。羧基端76个氨基酸没有显著的相似性（20%）。单克隆抗体V，其识别该羧基末端部分的表位，在水螅和哺乳动物细胞中的Western印迹中与33 kDa的蛋白质反应，而不与45 kDa的“层粘连蛋白结合蛋白”反应。33 kDa的蛋白质不是细胞外或跨膜的，但具有严格的细胞内位置，如其氨基酸序列和免疫细胞化学和细胞分级研究所示。在非分裂的哺乳动物细胞中，33 kDa蛋白与丝状结构共定位;在分裂的细胞中，它与丝状结构解离并集中。存在的SPLR序列，这是p34 cdc 2激酶的共识磷酸化基序，连接这个33 kDa的蛋白质在细胞周期中发生的事件。
In hydra and in mammalian cells the monoclonal antibody V recognises an epitope which colocalises with cytoskeletal structures. Using this antibody for expression screening, a cDNA clone (955 bp) was isolated from hydra, which covers an open reading frame for a protein of 294 amino acids with a calculated molecular mass of 32.8 kDa. Northern blot analysis of hydra RNA resulted in a single mRNA species of 1.2 kb, and primer extension experiments proved this to be the full length message. 218 residues at the amino terminus of the hydra protein show extensive homology (73.5%) to a human protein designated 'laminin binding protein'. The carboxyl-terminal 76 amino acids possess no significant similarity (20%). The monoclonal antibody V, which recognises an epitope in this carboxyl-terminal part, reacts in Western blots, both in hydra and in mammalian cells, with a protein of 33 kDa and not with the 45 kDa 'laminin binding protein'. The 33 kDa protein is not extracellular or transmembrane, but has a strictly intracellular location as indicated by its amino acid sequence and by immunocytochemical and cell fractionation studies. In non-dividing mammalian cells the 33 kDa protein colocalises with filamentous structures; in dividing cells it dissociates from it and concentrates centrally. Presence of the SPLR-sequence, which is the consensus phosphorylation motif for the p34cdc2 kinase, links this 33 kDa protein to events occurring during the cell cycle.
由单克隆抗体定义的寡水螅神经网络中的细胞子集：其排列和发育。
影响因子：
2.7
作者：
Dunne,JF;Javois,LC;Huang,LW;Bode,HR
通讯作者：
Bode,HR
Spisula Solidissima 卵母细胞核膜的减数分裂破坏涉及核纤层蛋白的磷酸化和释放。
影响因子：
2.7
作者：
Dessev,G;Goldman,R
通讯作者：
Goldman,R