Distinctive gut microbiota of honey bees assessed using deep sampling from individual worker bees.
Distinctive gut microbiota of honey bees assessed using deep sampling from individual worker bees.
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DOI：
10.1371/journal.pone.0036393
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Sabree ZL
中科院分区：
文献类型：
--
作者：
Moran NA;Hansen AK;Powell JE;Sabree ZL
对蜜蜂（Apis mellifera）的16S rDNA序列的调查显示，成年工蜂肠道中存在8种不同的细菌类型。由于以前的研究仅限于来自多个宿主的样本的相对较少的序列，因此在菌落和位置内以及菌落和位置之间的个体之间这种微生物群的变异程度尚不清楚。我们调查了来自美国亚利桑那州和马里兰州两个地点的40名工人的肠道微生物群，每个地点取样四个菌落。使用通用引物扩增16S核糖体RNA基因的区域，并使用454 pyrotag方法对扩增子进行测序，从而能够分析约330，000个细菌读数。超过99%的这些序列属于集群的第一个blaeblasthits在GenBank中的成员已知的蜜蜂的蜜蜂类型。每只蜜蜂都有四种类型的芽孢杆菌，一种在γ变形菌（对应于“Gilldatus Gilpella apicola”）中，一种在β变形菌（“Gilldatus Sillpella alvi”）中，两种在乳杆菌中，尽管它们的频率各不相同。在所有菌落和两个部位均存在相同的典型细菌菌落类型。社区配置文件显着不同的殖民地和网站之间，主要是由于在亚利桑那州的一些殖民地的两种肠杆菌没有检索以前从蜜蜂。分析的桑格序列的核糖体RNA的Sagrassella和Gilgelella的类型揭示了单一的蜜蜂包含许多不同的菌株的每一种类型。菌株表现出一定的分化的地方，特别是对Sassgrassella的类型。
Surveys of 16S rDNA sequences from the honey bee, Apis mellifera, have revealed the presence of eight distinctive bacterial phylotypes in intestinal tracts of adult worker bees. Because previous studies have been limited to relatively few sequences from samples pooled from multiple hosts, the extent of variation in this microbiota among individuals within and between colonies and locations has been unclear. We surveyed the gut microbiota of 40 individual workers from two sites, Arizona and Maryland USA, sampling four colonies per site. Universal primers were used to amplify regions of 16S ribosomal RNA genes, and amplicons were sequenced using 454 pyrotag methods, enabling analysis of about 330,000 bacterial reads. Over 99% of these sequences belonged to clusters for which the first blastn hits in GenBank were members of the known bee phylotypes. Four phylotypes, one within Gammaproteobacteria (corresponding to “Candidatus Gilliamella apicola”) one within Betaproteobacteria (“Candidatus Snodgrassella alvi”), and two within Lactobacillus, were present in every bee, though their frequencies varied. The same typical bacterial phylotypes were present in all colonies and at both sites. Community profiles differed significantly among colonies and between sites, mostly due to the presence in some Arizona colonies of two species of Enterobacteriaceae not retrieved previously from bees. Analysis of Sanger sequences of rRNA of the Snodgrassella and Gilliamella phylotypes revealed that single bees contain numerous distinct strains of each phylotype. Strains showed some differentiation between localities, especially for the Snodgrassella phylotype.
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影响因子：
4.9
作者：
Martinson, Vincent G.;Danforth, Bryan N.;Moran, Nancy A.
通讯作者：
Moran, Nancy A.
影响因子：
4.2
作者：
Babendreier, Dirk;Joller, David;Widmer, Franco
通讯作者：
Widmer, Franco
影响因子：
4.4
作者：
Corby-Harris, Vanessa;Pontaroli, Ana Clara;Promislow, Daniel E. L.
通讯作者：
Promislow, Daniel E. L.
影响因子：
64.8
作者：
Kau, Andrew L.;Ahern, Philip P.;Griffin, Nicholas W.;Goodman, Andrew L.;Gordon, Jeffrey I.
通讯作者：
Gordon, Jeffrey I.
影响因子：
4.4
作者：
Broderick, NA;Raffa, KF;Handelsman, J
通讯作者：
Handelsman, J