Mmf1p, a Novel Yeast Mitochondrial Protein Conserved throughout Evolution and Involved in Maintenance of the Mitochondrial Genome
Mmf1p, a Novel Yeast Mitochondrial Protein Conserved throughout Evolution and Involved in Maintenance of the Mitochondrial Genome
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Mmf1p，一种在整个进化过程中保守的新型酵母线粒体蛋白，参与线粒体基因组的维护
DOI：
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发表时间：
2000
影响因子：
5.3
通讯作者：
M. Tommasino
中科院分区：
文献类型：
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作者：
Ellinor Oxelmark;A. Marchini;I. Malanchi;F. Magherini;L. Jaquet;M. A. Nasser Hajibagheri;Kenneth J. Blight;J. Jauniaux;M. Tommasino
最近发现了一个在整个进化过程中高度保守的新蛋白家族（p14.5，或YERO57c/YJGFc）。这些蛋白质的生物学作用尚未被很好地描述。p14.5家族的两个成员存在于酿酒酵母中。在这项研究中，我们对这两种酵母蛋白的一些生物学功能进行了表征。Mmf1p是一种线粒体基质因子，同源的Mmf1p因子（Hmf1p）可与可溶性细胞质组分结合。Δmmf1cells失去线粒体DNA (mtDNA)，生长速度下降，而Δhmf1细胞不显示任何可见的表型。此外，我们通过遗传分析证明Mmf1p在mtDNA的复制和分离中不起直接作用。Rho + Δmmf1单倍体细胞可以在含有不可发酵碳源的培养基上直接解剖四分体。我们的数据还表明，Mmf1p和Hmf1p在不同的亚细胞区室中具有相似的生物学功能。当Hmf1p与Mmf1p先导肽融合时，被运输到线粒体中，并能够在功能上取代Mmf1p。此外，我们发现同源哺乳动物蛋白在功能上与Mmf1p相关。人类p14.5定位于酵母线粒体并挽救Δmmf1-associated表型。此外，大鼠肝脏线粒体的分离表明，大鼠p14.5与Mmf1p一样，是基质的可溶性蛋白。我们的研究确定了Mmf1p的生物学功能，并进一步表明这种功能在p14.5家族成员之间是保守的。
ABSTRACT A novel protein family (p14.5, or YERO57c/YJGFc) highly conserved throughout evolution has recently been identified. The biological role of these proteins is not yet well characterized. Two members of the p14.5 family are present in the yeast Saccharomyces cerevisiae. In this study, we have characterized some of the biological functions of the two yeast proteins. Mmf1p is a mitochondrial matrix factor, and homologous Mmf1p factor (Hmf1p) copurifies with the soluble cytoplasmic fraction. Δmmf1cells lose mitochondrial DNA (mtDNA) and have a decreased growth rate, while Δhmf1 cells do not display any visible phenotype. Furthermore, we demonstrate by genetic analysis that Mmf1p does not play a direct role in replication and segregation of the mtDNA. rho+ Δmmf1 haploid cells can be obtained when tetrads are directly dissected on medium containing a nonfermentable carbon source. Our data also indicate that Mmf1p and Hmf1p have similar biological functions in different subcellular compartments. Hmf1p, when fused with the Mmf1p leader peptide, is transported into mitochondria and is able to functionally replace Mmf1p. Moreover, we show that homologous mammalian proteins are functionally related to Mmf1p. Human p14.5 localizes in yeast mitochondria and rescues the Δmmf1-associated phenotypes. In addition, fractionation of rat liver mitochondria showed that rat p14.5, like Mmf1p, is a soluble protein of the matrix. Our study identifies a biological function for Mmf1p and furthermore indicates that this function is conserved between members of the p14.5 family.
HMG1 样酵母线粒体组蛋白 HM 和细菌组蛋白样蛋白 HU 之间的功能互补。
DOI：
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发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Megraw,TL;Chae,CB
通讯作者：
Chae,CB
来自酵母线粒体的 HMG1 相关蛋白的 DNA 结合特性。
DOI：
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发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Diffley,JF;Stillman,B
通讯作者：
Stillman,B
DOI：
10.1073/pnas.85.7.2120
发表时间：
1988-04-01
影响因子：
11.1
作者：
DIFFLEY, JFX;STILLMAN, B
通讯作者：
STILLMAN, B