Pectic homogalacturonan masks abundant sets of xyloglucan epitopes in plant cell walls.
Pectic homogalacturonan masks abundant sets of xyloglucan epitopes in plant cell walls.
复制标题
果皮同质分离剂掩盖了植物细胞壁中丰富的木葡聚糖表位。
DOI：
10.1186/1471-2229-8-60
复制
发表时间：
2008-05-22
影响因子：
5.3
通讯作者：
Knox JP
中科院分区：
文献类型：
--
作者：
Marcus SE;Verhertbruggen Y;Hervé C;Ordaz-Ortiz JJ;Farkas V;Pedersen HL;Willats WG;Knox JP
需要分子探针来原位检测细胞壁聚合物，以帮助了解其细胞生物学，并且多项研究表明，细胞壁表位在植物器官各部分的出现受到限制，表明细胞壁结构在发育过程中受到高度调控。木葡聚糖是双子叶植物初生细胞壁的主要半纤维素或交联聚糖，但对其与细胞发育和细胞壁微观结构相关的存在或功能知之甚少。使用新糖蛋白方法，将罗望子种子木葡聚糖的 XXXG 七糖与 BSA 偶联以产生免疫原，我们生成了针对木葡聚糖 XXXG 结构基序的大鼠单克隆抗体（命名为 LM15）。 LM15 的特异性已通过使用聚糖微阵列分析 LM15 结合和寡糖半抗原结合抑制研究得到证实。使用 LM15 分析罗望子和旱金莲种子细胞壁中的木葡聚糖（其中木葡聚糖作为储存多糖存在）表明 LM15 木葡聚糖表位存在于整个罗望子种子的增厚细胞壁以及邻近中层的罗望子种子增厚细胞壁的外部区域。 旱金莲种子。 LM15 与烟草和豌豆茎节间切片结合的免疫荧光分析表明，木葡聚糖表位仅限于这些器官中的几种细胞类型。从同等切片中酶促去除果胶同型半乳糖醛酸，导致在这些器官中大量检测到 LM15 木葡聚糖表位的不同模式，以及与一系列细胞类型的细胞壁微结构相关的多样性。这些观察结果支持木葡聚糖与植物细胞壁中的果胶相关的观点。他们还表明，与细胞发育和细胞分化相关的细胞壁表位的记录模式可能需要重新考虑与其他细胞壁成分对细胞壁表位的潜在掩蔽的关系。
Molecular probes are required to detect cell wall polymers in-situ to aid understanding of their cell biology and several studies have shown that cell wall epitopes have restricted occurrences across sections of plant organs indicating that cell wall structure is highly developmentally regulated. Xyloglucan is the major hemicellulose or cross-linking glycan of the primary cell walls of dicotyledons although little is known of its occurrence or functions in relation to cell development and cell wall microstructure. Using a neoglycoprotein approach, in which a XXXG heptasaccharide of tamarind seed xyloglucan was coupled to BSA to produce an immunogen, we have generated a rat monoclonal antibody (designated LM15) to the XXXG structural motif of xyloglucans. The specificity of LM15 has been confirmed by the analysis of LM15 binding using glycan microarrays and oligosaccharide hapten inhibition of binding studies. The use of LM15 for the analysis of xyloglucan in the cell walls of tamarind and nasturtium seeds, in which xyloglucan occurs as a storage polysaccharide, indicated that the LM15 xyloglucan epitope occurs throughout the thickened cell walls of the tamarind seed and in the outer regions, adjacent to middle lamellae, of the thickened cell walls of the nasturtium seed. Immunofluorescence analysis of LM15 binding to sections of tobacco and pea stem internodes indicated that the xyloglucan epitope was restricted to a few cell types in these organs. Enzymatic removal of pectic homogalacturonan from equivalent sections resulted in the abundant detection of distinct patterns of the LM15 xyloglucan epitope across these organs and a diversity of occurrences in relation to the cell wall microstructure of a range of cell types. These observations support ideas that xyloglucan is associated with pectin in plant cell walls. They also indicate that documented patterns of cell wall epitopes in relation to cell development and cell differentiation may need to be re-considered in relation to the potential masking of cell wall epitopes by other cell wall components.
登录
查看更多内容
影响因子：
6.5
作者：
Buckeridge, MS;dos Santos, HP;Tiné, MAS
通讯作者：
Tiné, MAS
影响因子：
4.1
作者：
Brown, IE;Mallen, MH;Black, GW
通讯作者：
Black, GW
影响因子：
7.4
作者：
Freshour, G;Bonin, CP;Hahn, MG
通讯作者：
Hahn, MG
影响因子：
7.4
作者：
Desveaux, D;Faik, A;Maclachlan, G
通讯作者：
Maclachlan, G
影响因子：
7.4
作者：
Nguema-Ona, E;Andème-Onzighi, C;Driouich, A
通讯作者：
Driouich, A