Magnetic Particle Imaging for Highly Sensitive, Quantitative, and Safe in Vivo Gut Bleed Detection in a Murine Model.
Magnetic Particle Imaging for Highly Sensitive, Quantitative, and Safe in Vivo Gut Bleed Detection in a Murine Model.
复制标题
DOI：
10.1021/acsnano.7b04844
复制
发表时间：
2017-12-26
期刊：
影响因子：
17.1
通讯作者：
Conolly SM
中科院分区：
文献类型：
--
作者：
Yu EY;Chandrasekharan P;Berzon R;Tay ZW;Zhou XY;Khandhar AP;Ferguson RM;Kemp SJ;Zheng B;Goodwill PW;Wendland MF;Krishnan KM;Behr S;Carter J;Conolly SM
在美国，胃肠道（GI）出血每年导致超过30万例住院治疗。成像在准确定位出血源以及时干预方面起着至关重要的作用。磁粒子成像（MPI）是一种新兴的临床可翻译的成像模式，其以非凡的对比度和灵敏度对超顺磁性氧化铁（SPIO）示踪剂进行成像。该线性定量模态具有零背景组织信号和零信号深度衰减。MPI也是安全的：患者的电离辐射暴露为零，并且临床批准的示踪剂可以与MPI一起使用。在这项研究中，我们证明了MPI与长循环、PEG稳定的SPIO一起沿着用于快速体内检测和定量胃肠道出血。本研究使用遗传上易发生GI息肉（ApcMin/+）的小鼠模型，并使用肝素作为抗凝剂诱导急性GI出血。然后，我们通过尾静脉注射MPI定制的长循环SPIO，并使用我们定制的高分辨率无场线（FFL）MPI扫描仪随时间跟踪示踪剂生物分布。动态MPI投影图像捕获了下胃肠道中的示踪剂积聚，对比度极佳。MPI图像的定量分析表明，小鼠的GI出血率在1至5 μL/min之间。尽管目前没有人类规模的MPI系统，并且MPI定制的SPIO需要进行进一步的开发和评估，但该技术的临床转化是可以实现的。强大的对比度，灵敏度，安全性，在身体任何地方成像的能力，沿着长循环SPIO，使MPI成为GI出血的临床诊断工具。
Gastrointestinal (GI) bleeding causes more than 300,000 hospitalizations per year in the United States. Imaging plays a crucial role in accurately locating the source of the bleed for timely intervention. Magnetic Particle Imaging (MPI) is an emerging clinically translatable imaging modality that images superparamagnetic iron-oxide (SPIO) tracers with extraordinary contrast and sensitivity. This linearly quantitative modality has zero background tissue signal and zero signal depth attenuation. MPI is also safe: there is zero ionizing radiation exposure to the patient and clinically approved tracers can be used with MPI. In this study, we demonstrate the use of MPI along with long-circulating, PEG-stabilized SPIOs for rapid in vivo detection and quantification of GI bleed. A mouse model genetically predisposed to GI polyp development (ApcMin/+) was used for this study, and heparin was used as an anticoagulant to induce acute GI bleeding. We then injected MPI-tailored, long-circulating SPIOs through the tail vein, and tracked the tracer biodistribution over time using our custom-built high resolution field-free line (FFL) MPI scanner. Dynamic MPI projection images captured tracer accumulation in the lower GI tract with excellent contrast. Quantitative analysis of the MPI images show that the mice experienced GI bleed rates between 1 and 5 μL/min. Although there are currently no human scale MPI systems, and MPI-tailored SPIOs need to undergo further development and evaluation, clinical translation of the technique is achievable. The robust contrast, sensitivity, safety, ability to image anywhere in the body, along with long-circulating SPIOs lends MPI outstanding promise as a clinical diagnostic tool for GI bleeding.
登录
查看更多内容
影响因子：
3.5
作者：
Keselman P;Yu EY;Zhou XY;Goodwill PW;Chandrasekharan P;Ferguson RM;Khandhar AP;Kemp SJ;Krishnan KM;Zheng B;Conolly SM
通讯作者：
Conolly SM
影响因子：
3.7
作者：
Antonelli, Antonella;Sfara, Carla;Magnani, Mauro
通讯作者：
Magnani, Mauro
影响因子：
6.7
作者：
Khandhar AP;Keselman P;Kemp SJ;Ferguson RM;Goodwill PW;Conolly SM;Krishnan KM
通讯作者：
Krishnan KM
DOI：
10.2214/ajr.15.14534
发表时间：
2015-09
期刊：
AJR. American journal of roentgenology
影响因子：
--
作者：
Hope MD;Hope TA;Zhu C;Faraji F;Haraldsson H;Ordovas KG;Saloner D
通讯作者：
Saloner D
影响因子：
3.8
作者：
Ferguson, R. Matthew;Minard, Kevin R.;Krishnan, Kannan M.
通讯作者：
Krishnan, Kannan M.