Characterization of oriented protein-ceramic and protein-polymer-composites for cartilage tissue engineering using synchrotron μ-CT
Characterization of oriented protein-ceramic and protein-polymer-composites for cartilage tissue engineering using synchrotron μ-CT
复制标题
使用同步加速器 μ-CT 表征软骨组织工程用定向蛋白陶瓷和蛋白聚合物复合材料
DOI：
10.3139/146.101509
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发表时间：
2007
影响因子：
0.8
通讯作者：
H. Schubert
中科院分区：
文献类型：
--
作者：
R. Zehbe;A. Haibel;C. Brochhausen;U. Gross;C. Kirkpatrick;H. Schubert
摘要本文报道了三种不同的明胶基支架材料的合成，用于关节软骨缺损的重建。第一种支架设计基于未改性的定向明胶网络，而第二种设计还包括附着的无机羟基磷灰石层，第三种设计包括聚（1-丙交酯）微球作为未来药物释放应用的模型材料。使用同步加速器μ-CT对所有三种支架设计进行表征和成像，获得先前定义的样品区域的完整体积重建。此外，两个未修饰的支架与猪软骨细胞一起培养一周。然后，使用Au-赖氨酸和银增强剂的组合标记附着的细胞。在同步加速器μ-CT分析中，由于标记细胞和未标记支架在几毫米体积内的X射线吸收差异，我们因此能够绘制细胞分布。
Abstract In this paper we report on the synthesis of three different gelatine based scaffold materials for the reconstruction of articular cartilage defects. The first scaffold design is based on an unmodified, oriented gelatine network, while the second design further comprises an attached inorganic hydroxyapatite layer and the third design includes poly(l-lactide) microspheres as a model material for future drug-release applications. All three scaffold designs were characterized and imaged using synchrotron μ-CT, obtaining a complete volumetric reconstruction of a previously defined sample region. Furthermore, two unmodified scaffolds were cultivated for one week with porcine chondrocytes. Afterwards the attached cells were labelled using a combination of Au-lysine and silver enhancer. In synchrotron μ-CT analysis we were thus able to map the cell distribution due to the difference in X-ray absorption of the labelled cells and the non labelled scaffolds in a volume of several millimetres.
影响因子：
2.4
作者：
Wang, JHC;Jia, FY;Woo, SLY
通讯作者：
Woo, SLY
影响因子：
4.9
作者：
Rajagopalan, S;Lu, LC;Robb, RA
通讯作者：
Robb, RA