HFR1 is crucial for transcriptome regulation in the cryptochrome 1-mediated early response to blue light in Arabidopsis thaliana.
HFR1 is crucial for transcriptome regulation in the cryptochrome 1-mediated early response to blue light in Arabidopsis thaliana.
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DOI：
10.1371/journal.pone.0003563
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发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Liu D
中科院分区：
文献类型：
--
作者：
Zhang XN;Wu Y;Tobias JW;Brunk BP;Deitzer GF;Liu D
隐色素是参与发育和生物钟调节的蓝光光感受器。它们作为光传感器存在于真核生物和原核生物中。远红1中的长下胚轴（Long Hypocotyl in Far-Red 1, HFR1）在植物蓝光和远红光信号传导中都是一个正调控因子和可能的转录因子。然而，HFR1在隐色素1 （cry1）介导的蓝光信号传导中调控的基因靶点尚未得到全球的解决。我们以cry1和hfr1依赖的方式检测了对1小时蓝光的反应的转录组谱。引人注目的是，在蓝光诱导的hfr1依赖性基因中，超过70%依赖于cry1，反之亦然。在这些基因中发现w -box和OCS元件的高代表性，表明cry1和HFR1对基因表达的强烈共同调控可能是通过这两个顺式元件。我们还发现，cry1是维持蓝光下HFR1蛋白水平所必需的，并且HFR1蛋白水平与整体基因表达模式密切相关。综上所述，在cry1介导的早期蓝光信号中，HFR1对调控全局基因表达至关重要，特别是对含有w -box和OCS元件的基因的功能。
Cryptochromes are blue light photoreceptors involved in development and circadian clock regulation. They are found in both eukaryotes and prokaryotes as light sensors. Long Hypocotyl in Far-Red 1 (HFR1) has been identified as a positive regulator and a possible transcription factor in both blue and far-red light signaling in plants. However, the gene targets that are regulated by HFR1 in cryptochrome 1 (cry1)-mediated blue light signaling have not been globally addressed. We examined the transcriptome profiles in a cry1- and HFR1-dependent manner in response to 1 hour of blue light. Strikingly, more than 70% of the genes induced by blue light in an HFR1-dependent manner were dependent on cry1, and vice versa. High overrepresentation of W-boxes and OCS elements were found in these genes, indicating that this strong cry1 and HFR1 co-regulation on gene expression is possibly through these two cis-elements. We also found that cry1 was required for maintaining the HFR1 protein level in blue light, and that the HFR1 protein level is strongly correlated with the global gene expression pattern. In summary, HFR1, which is fine-tuned by cry1, is crucial for regulating global gene expression in cry1-mediated early blue light signaling, especially for the function of genes containing W-boxes and OCS elements.
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