Genetic alterations on chromosome 16 and 17 are important features of ductal carcinoma in situ of the breast and are associated with histologic type.
Genetic alterations on chromosome 16 and 17 are important features of ductal carcinoma in situ of the breast and are associated with histologic type.
复制标题
16和17染色体的遗传改变是导管癌的重要特征，与组织学类型有关。
DOI：
10.1038/sj.bjc.6693372
复制
发表时间：
1999-12
影响因子：
8.8
通讯作者：
van de Vijver, MJ
中科院分区：
文献类型：
--
作者：
Vos, CBJ;ter Haar, NT;Rosenberg, C;Peterse, JL;Cleton-Jansen, AM;Cornelisse, CJ;van de Vijver, MJ
我们通过微卫星分析（洛）、Southern印迹和比较基因组杂交（CGH）分析了导管原位癌（DCIS）中已知和假定的肿瘤抑制基因和癌基因位点的参与情况。共检测了78例单纯DCIS病例的洛性缺失，其中76个标记物分布于沿着所有染色体臂。与高分化DCIS（17%）相比，低分化DCIS（70%）中17号染色体上的洛更常见，而16号染色体上的缺失与高分化和中等分化DCIS相关（66%）。对于一个子集，我们做了Southern印迹和CGH分析。C-erbB 2/neu在低分化DCIS中的阳性率为30%。c-myc、mdm 2、bek、c-myc和表皮生长因子受体（EGF）均未扩增。CGH结果显示，低分化DCIS中最常见的改变是染色体8 q和17 q22 -24的增加和17 p的缺失，而高分化DCIS中则发现染色体1 q的扩增和16 q的缺失。我们的数据表明，染色体16 q上一个未知的肿瘤抑制基因的失活与大多数分化良好和中等分化的DCIS的发生有关，17号染色体上各种基因的扩增和失活与低分化DCIS的发生有关。此外，这些数据表明，有一个遗传基础的分类DCIS在一个良好的和低分化的类型，并支持不同的遗传途径，以发展一个特定类型的乳腺原位癌的证据。© 1999癌症研究运动
We analysed the involvement of known and putative tumour suppressor- and oncogene loci in ductal carcinoma in situ (DCIS) by microsatellite analysis (LOH), Southern blotting and comparative genomic hybridization (CGH). A total of 78 pure DCIS cases, classified histologically as well, intermediately and poorly differentiated, were examined for LOH with 76 markers dispersed along all chromosome arms. LOH on chromosome 17 was more frequent in poorly differentiated DCIS (70%) compared to well-differentiated DCIS (17%), whereas loss on chromosome 16 was associated with well- and intermediately differentiated DCIS (66%). For a subset we have done Southern blot- and CGH analysis. C-erbB2/neu was amplified in 30% of poorly differentiated DCIS. No amplification was found of c-myc, mdm2, bek, flg and the epidermal growth factor (EGF)-receptor. By CGH, most frequent alterations in poorly differentiated DCIS were gains on 8q and 17q22–24 and deletion on 17p, whereas in well-differentiated DCIS amplification on chromosome 1q and deletion on 16q were found. In conclusion, our data indicates that inactivation of a yet unknown tumour suppressor gene on chromosome 16q is implicated in the development of most well and intermediately differentiated DCIS whereas amplification and inactivation of various genes on chromosome 17 are implicated in the development of poorly differentiated DCIS. Furthermore these data show that there is a genetic basis for the classification of DCIS in a well and poorly differentiated type and support the evidence of different genetic routes to develop a specific type of carcinoma in situ of the breast. © 1999 Cancer Research Campaign
登录
查看更多内容
影响因子：
8
作者：
James, LA;Mitchell, ELD;Varley, JM
通讯作者：
Varley, JM
影响因子：
11.4
作者：
Berx, G;CletonJansen, AM;vanRoy, F
通讯作者：
vanRoy, F
DOI：
10.1111/j.1349-7006.1994.tb02902.x
发表时间：
1994-10-01
期刊：
JAPANESE JOURNAL OF CANCER RESEARCH
影响因子：
--
作者：
KANAI, Y;ODA, T;HIROHASHI, S
通讯作者：
HIROHASHI, S
影响因子：
64.8
作者：
OLINER, JD;KINZLER, KW;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
影响因子：
64.5
作者：
LIVINGSTONE, LR;WHITE, A;TISTY, TD
通讯作者：
TISTY, TD