Effect of salivary agglutination on oral streptococcal clearance by human polymorphonuclear neutrophil granulocytes.
Effect of salivary agglutination on oral streptococcal clearance by human polymorphonuclear neutrophil granulocytes.
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DOI：
10.1111/omi.12164
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发表时间：
2017-06
影响因子：
3.7
通讯作者：
Kreth J
中科院分区：
文献类型：
--
作者：
Itzek A;Chen Z;Merritt J;Kreth J
唾液凝集是一种重要的宿主防御机制，可以聚集口腔共生菌和入侵病原体。与单个细胞相比，唾液流动和随后的吞咽更容易清除聚集的细菌。通过多形核中性粒细胞对细菌的吞噬清除似乎也随着细菌聚集物的大小而在一定程度上增加。为了确定唾液凝集与宿主吞噬先天免疫反应之间的联系，我们开发了一种体外凝集试验，再现了唾液细菌聚集体的平均大小。以口腔共生戈登链球菌为模型生物，研究了唾液凝集对多形核中性粒细胞吞噬廓清的影响。在这里，我们描述了唾液中的godonii聚集体很容易通过吞噬作用被清除，而单个细菌细胞在被吞噬和杀死方面表现出明显的延迟。此外，在吞噬之前，多形核中性粒细胞能够诱导特异性的去聚集，这取决于丝氨酸蛋白酶的活性。本文提出的数据表明，唾液的细菌细胞凝集导致一个理想的大小，以供多形核中性粒细胞识别。作为第一道防线，这些吞噬细胞能够识别聚集体，并通过丝氨酸蛋白酶将聚集体分解成更易于控制的大小，从而有效地吞噬并随后在吞噬酶体中杀死它们。这种观察到的机制不仅可以防止口腔细菌细胞在进入血液时迅速扩散，还可以避免相关多形核中性粒细胞的脱粒，从而防止对附近组织的附带损伤。
Salivary agglutination is an important host defense mechanism to aggregate oral commensal bacteria as well as invading pathogens. Saliva flow and subsequent swallowing more easily clear aggregated bacteria compared to single cells. Phagocytic clearance of bacteria through polymorphonuclear neutrophil granulocytes also seems to increase to a certain extent with the size of bacterial aggregates. To determine a connection between salivary agglutination and the host innate immune response by phagocytosis, an in vitro agglutination assay was developed reproducing the average size of salivary bacterial aggregates. Using the oral commensal Streptococcus gordonii as a model organism, the effect of salivary agglutination to the phagocytic clearance through polymorphonuclear neutrophil granulocytes was investigated. Here we describe that salivary aggregates of S. gordonii are readily cleared through phagocytosis, while single bacterial cells showed a significant delay in being phagocytosed and killed. Furthermore, prior to phagocytosis the polymorphonuclear neutrophil granulocytes were able to induce a specific de-aggregation, which was dependent on serine protease activity. The herein presented data suggest that salivary agglutination of bacterial cells leads to an ideal size for recognition by polymorphonuclear neutrophil granulocytes. As a first line of defense, these phagocytic cells are able to recognize the aggregates and de-aggregate them via serine proteases to a more manageable size for efficient phagocytosis and subsequent killing in the phagolysosome. This observed mechanism not only prevents the rapid spreading of oral bacterial cells while entering the bloodstream but would also avoid degranulation of involved polymorphonuclear neutrophil granulocytes thus preventing collateral damage to nearby tissue.
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影响因子：
3.1
作者：
Lee, Si Young;Cisar, John O.;Sandberg, Ann L.
通讯作者：
Sandberg, Ann L.
影响因子：
1.5
作者：
Lick, SD;Edozie, SN;Conti, VR
通讯作者：
Conti, VR
影响因子：
4.7
作者：
Morris, James A.;Harrison, Linda M.;Telford, David R.
通讯作者：
Telford, David R.
影响因子：
3.1
作者：
Loimaranta, V;Jakubovics, NS;Strömberg, N
通讯作者：
Strömberg, N
影响因子：
30.3
作者：
Dalia AB;Weiser JN
通讯作者：
Weiser JN