Lamin A, lamin B, and lamin B receptor analogues in yeast
Lamin A, lamin B, and lamin B receptor analogues in yeast
复制标题
酵母中的核纤层蛋白 A、核纤层蛋白 B 和核纤层蛋白 B 受体类似物
作者：
S. Georgatos;I. Maroulakou;Gfinter Blobel;Worman H J;J. Yuan;G. Blobel
先前的研究表明，火鸡红细胞核纤层蛋白 B 通过称为 p58 或核纤层蛋白 B 受体的 58-kD 整合膜蛋白锚定到核膜上（Worman H. J.、J. Yuan、G. Blobel 和 S. D. Georgatos. 1988. Proc. Natl. Acad. Sci. USA. 85:8531-8534）。我们现在在酿酒酵母中鉴定出 p58 类似物。火鸡红细胞核纤层蛋白 B 以高亲和力与酵母尿素提取的核膜结合，主要与 58 kD 多肽结合。这种酵母多肽被针对火鸡 p58 的多克隆抗体识别，完全与核部分分开，在核膜的尿素提取后保持膜结合，并且根据肽图谱标准在结构上与火鸡 p58 相似。使用针对火鸡红细胞核纤层蛋白 A 和 B 的多克隆抗体，我们还鉴定了两种酵母核纤层蛋白形式。酵母核纤层蛋白 B 类似物的分子量为 66 kD，在结构上与红细胞核纤层蛋白 B 相关。此外，酵母核纤层蛋白 B 类似物仅与核膜部分分开，通过尿素提取从包膜中定量去除，并与火鸡核纤层蛋白 A 和波形蛋白结合。与许多高等真核核纤层蛋白 B 形式一样，酵母类似物在化学上是异质的，包含两个具有不同电荷特性的血清学相关物种。火鸡核纤层蛋白 A 的抗体检测到 74 kD 的酵母蛋白，略大于火鸡核纤层蛋白 A。它比酵母核纤层蛋白 B 类似物更丰富，并且在可溶性细胞质部分和核膜部分之间进行分配。酵母核纤层蛋白 A 类似物可以通过尿素从核膜中提取，显示出与火鸡和大鼠核纤层蛋白 A 的结构相似性，并与分离的火鸡核纤层蛋白 B 结合。这些数据表明，典型核纤层成分（核纤层蛋白 A 和 B，以及核纤层蛋白 B 受体）的类似物存在于酵母中，并且其行为与其脊椎动物对应物相同。
Previous studies have shown that turkey erythrocyte lamin B is anchored to the nuclear envelope via a 58-kD integral membrane protein termed p58 or lamin B receptor (Worman H. J., J. Yuan, G. Blobel, and S. D. Georgatos. 1988. Proc. Natl. Acad. Sci. USA. 85:8531-8534). We now identify a p58 analogue in the yeast Saccharomyces cerevisiae. Turkey erythrocyte lamin B binds to yeast urea-extracted nuclear envelopes with high affinity, associating predominantly with a 58-kD polypeptide. This yeast polypeptide is recognized by polyclonal antibodies against turkey p58, partitions entirely with the nuclear fraction, remains membrane bound after urea extraction of the nuclear envelopes, and is structurally similar to turkey p58 by peptide mapping criteria. Using polyclonal antibodies against turkey erythrocyte lamins A and B, we also identify two yeast lamin forms. The yeast lamin B analogue has a molecular mass of 66 kD and is structurally related to erythrocyte lamin B. Moreover, the yeast lamin B analogue partitions exclusively with the nuclear envelope fraction, is quantitatively removed from the envelopes by urea extraction, and binds to turkey lamin A and vimentin. As many higher eukaryotic lamin B forms, the yeast analogue is chemically heterogeneous comprising two serologically related species with different charge characteristics. Antibodies against turkey lamin A detect a 74-kD yeast protein, slightly larger than the turkey lamin A. It is more abundant than the yeast lamin B analogue and partitions between a soluble cytoplasmic fraction and a nuclear envelope fraction. The yeast lamin A analogue can be extracted from the nuclear envelope by urea, shows structural similarity to turkey and rat lamin A, and binds to isolated turkey lamin B. These data indicate that analogues of typical nuclear lamina components (lamins A and B, as well as lamin B receptor) are present in yeast and behave as their vertebrate counterparts.
核膜中的核纤层蛋白 B 受体。
DOI：
10.1073/pnas.85.22.8531
发表时间：
1988
影响因子：
11.1
作者：
Worman,HJ;Yuan,J;Blobel,G;Georgatos,SD
通讯作者：
Georgatos,SD
DOI：
10.1016/s0021-9258(18)37903-1
发表时间：
1988-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Worman Hj;I. Lazaridis;Georgatos Sd
通讯作者：
Worman Hj;I. Lazaridis;Georgatos Sd