Polymerase chain reaction-based enzyme immunoassay for quantitation of telomerase activity: application to colorectal cancers.
Polymerase chain reaction-based enzyme immunoassay for quantitation of telomerase activity: application to colorectal cancers.
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DOI：
10.1111/j.1349-7006.1999.tb00745.x
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发表时间：
1999-03
期刊：
影响因子：
--
通讯作者：
Wang TC
中科院分区：
文献类型：
--
作者：
Cheng AJ;Tang R;Wang JY;Chang JT;Wang TC
端粒酶是一种专门的逆转录酶，将端粒序列合成到人类染色体末端。它似乎存在于大多数原发性人类癌症组织中，并且可能具有作为通用肿瘤标志物的潜力。在这份报告中，我们描述了一种灵敏的，非放射性的，基于聚合酶链反应（PCR）的酶免疫测定（EIA），用于定量人细胞中端粒酶活性。该PCR-EIA非常方便，可以在3小时内轻松完成。在4种不同的细胞系上测得的PCR-EIA结果与传统端粒重复序列扩增方法（TRAP）结果之间的相关系数超过0.98。对该方法的临床应用进行了评价，并与传统的TRAP方法的结果进行了比较。我们的数据表明，传统的TRAP和PCR-EIA测量的端粒酶活性高度相关，我们建议PCR-EIA方法可以取代传统的TRAP。
Telomerase is a specialized reverse transcriptase that synthesizes telomeric sequences onto human chromosomal ends. It appears to be present in the majority of primary human cancer tissues, and may have potential as a universal tumor marker. In this report, we describe a sensitive, non‐radioactive, polymerase chain reaction (PCR)‐based enzyme immunoassay (EIA) for the quantitation of telomerase activity in human cells. This PCR‐EIA is convenient and can be easily completed within 3 h. The correlation coefficient between the results of PCR‐EIA and the conventional telomeric repeat amplification protocol (TRAP) method, as measured on 4 different cell lines, was over 0.98. Evaluation of this method for clinical application was conducted with tissues obtained from patients with colorectal cancers and the results were compared with those of the conventional TRAP method. Our data indicate that telomerase activities measured by conventional TRAP and PCR‐EIA are highly correlated, and we suggest that the PCR‐EIA method can substitute for conventional TRAP.
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影响因子：
8.4
作者：
Morin, GB
通讯作者：
Morin, GB
DOI：
10.1093/jnci/90.4.316
发表时间：
1998-02-18
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
Cheng, AJ;Tang, RP;Wang, TCV
通讯作者：
Wang, TCV
影响因子：
8.4
作者：
Shay, JW;Bacchetti, S
通讯作者：
Bacchetti, S
影响因子：
56.9
作者：
Bodnar, AG;Ouellette, M;Wright, WE
通讯作者：
Wright, WE
影响因子：
8.4
作者：
Campisi, J
通讯作者：
Campisi, J