The Endothelial Glycocalyx and Retinal Hemodynamics.
The Endothelial Glycocalyx and Retinal Hemodynamics.
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内皮糖脂和视网膜血流动力学。
DOI：
10.3390/pathophysiology29040052
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发表时间：
2022-12-17
期刊：
影响因子：
--
通讯作者：
Harris, Norman R.
中科院分区：
文献类型：
--
作者：
Kaur, Gaganpreet;Leskova, Wendy;Harris, Norman R.
目的。先前的研究表明，内皮糖萼增加血管阻力，抑制血栓形成，对调节均匀血流和确保红细胞均匀分布至关重要。然而，糖萼的这些功能和后果尚未在视网膜中研究过。我们假设内皮糖萼是视网膜血流动力学和灌注的关键调节因子，并降低视网膜血栓形成的倾向。方法。小鼠注射内皮糖萼降解酶透明质酸酶和肝素酶。注射异硫氰酸-葡聚糖荧光素（2000 kDa）测定管腔直径，荧光标记红细胞测定红细胞速度和分布。直径和速度被用来计算视网膜血流量和剪切速率。平均循环时间是通过测量小动脉和静脉平均穿越时间的差值来计算的。注入玫瑰孟加拉染料，然后用绿光照射以诱导血栓形成。结果。急性输注透明质酸酶和肝素酶导致视网膜小动脉（7%）和静脉（16%）直径显著增加，并有增加小动脉速度的趋势。此外，降解导致静脉剪切速率显著降低（14%）。酶输注导致视网膜总血流量（26%）和视网膜微红细胞压积显著增加，但通过视网膜的平均循环时间没有变化。我们还观察到，随着糖萼的去除，视网膜血栓形成的倾向增强。结论。我们的数据表明，糖萼的急性降解可引起视网膜血流动力学的显著变化，血管直径、血流、微红细胞压积、促血栓条件增加，静脉剪切速率降低。
Purpose. Previous studies suggest that the endothelial glycocalyx adds to vascular resistance, inhibits thrombosis, and is critical for regulating homogeneous blood flow and ensuring uniform red blood cell (RBC) distribution. However, these functions and consequences of the glycocalyx have not been examined in the retina. We hypothesize that the endothelial glycocalyx is a critical regulator of retinal hemodynamics and perfusion and decreases the propensity for retinal thrombus formation. Methods. Hyaluronidase and heparinase, which are endothelial glycocalyx-degrading enzymes, were infused into mice. Fluorescein isothiocyanate–dextran (2000 kDa) was injected to measure lumen diameter, while RBC velocity and distribution were measured using fluorescently labeled RBCs. The diameters and velocities were used to calculate retinal blood flow and shear rates. Mean circulation time was calculated by measuring the difference between arteriolar and venular mean transit times. Rose Bengal dye was infused, followed by illumination with a green light to induce thrombosis. Results. The acute infusion of hyaluronidase and heparinase led to significant increases in both arteriolar (7%) and venular (16%) diameters in the retina, with a tendency towards increased arteriolar velocity. In addition, the degradation caused a significant decrease in the venular shear rate (14%). The enzyme infusion resulted in substantial increases in total retinal blood flow (26%) and retinal microhematocrit but no changes in the mean circulation time through the retina. We also observed an enhanced propensity for retinal thrombus formation with the removal of the glycocalyx. Conclusions. Our data suggest that acute degradation of the glycocalyx can cause significant changes in retinal hemodynamics, with increases in vessel diameter, blood flow, microhematocrit, pro-thrombotic conditions, and decreases in venular shear rate.
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影响因子：
17.8
作者：
Kur, Joanna;Newman, Eric A.;Chan-Ling, Tailoi
通讯作者：
Chan-Ling, Tailoi
影响因子：
4.6
作者：
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DOI：
10.1152/ajpheart.2001.280.3.h1051
发表时间：
2001-03-01
影响因子：
4.8
作者：
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通讯作者：
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影响因子：
3.1
作者：
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通讯作者：
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影响因子：
4.4
作者：
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通讯作者：
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