New method for GC/FID and GC-C-IRMS analysis of plasma free fatty acid concentration and isotopic enrichment.
New method for GC/FID and GC-C-IRMS analysis of plasma free fatty acid concentration and isotopic enrichment.
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DOI：
10.1016/j.jchromb.2008.08.009
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发表时间：
2008-09-15
期刊：
影响因子：
--
通讯作者：
Delany JP
中科院分区：
文献类型：
--
作者：
Kangani CO;Kelley DE;Delany JP
建立了一种简便、直接、准确的测定血浆中游离脂肪酸浓度和富集度的方法。的验证和常规方法的比较报告。三个酰胺衍生物，二甲基，二乙基和吡咯烷，进行了研究，以达到最佳的分辨率的个别脂肪酸。该方法涉及使用二甲胺/脱氧氟将血浆游离脂肪酸衍生为其二甲酰胺。该衍生化方法非常温和和有效，并且仅对游离脂肪酸具有选择性，因此不需要从总脂质提取物中分离。与甲酯衍生物相比，薄层色谱法后，直接法血浆中棕榈酸和硬脂酸浓度较低，油酸和亚油酸浓度升高。直接法测得的[13 C]棕榈酸酯同位素富集度显著高于BF 3/MeOH-TLC法测得的[13 C]棕榈酸酯同位素富集度。本方法提供了准确和精确的测量浓度以及富集时，气相色谱燃烧同位素比质谱分析。
A simple, direct and accurate method for the determination of concentration and enrichment of free fatty acids in human plasma was developed. The validation and comparison to a conventional method are reported. Three amide derivatives, dimethyl, diethyl and pyrrolidide, were investigated in order to achieve optimal resolution of the individual fatty acids. This method involves the use of dimethylamine/Deoxo-Fluor to derivatize plasma free fatty acids to their dimethylamides. This derivatization method is very mild and efficient, and is selective only towards free fatty acids so that no separation from a total lipid extract is required. The direct method gave lower concentrations for palmitic acid and stearic acid and increased concentrations for oleic acid and linoleic acid in plasma as compared to methylester derivative after thin-layer chromatography. The [13C]palmitate isotope enrichment measured using direct method was significantly higher than that observed with the BF3/MeOH-TLC method. The present method provided accurate and precise measures of concentration as well as enrichment when analyzed with gas chromatography combustion-isotope ratio-mass spectrometry.
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DOI：
10.1093/gerona/61.6.534
发表时间：
2006-06-01
影响因子：
5.1
作者：
Menshikova, Elizabeth V.;Ritov, Vladimir B.;Goodpaster, Bret H.
通讯作者：
Goodpaster, Bret H.
影响因子：
3.4
作者：
Hamilton, JTG;Christie, WW
通讯作者：
Christie, WW
影响因子：
1.9
作者：
ANDERSSON, BA;HEIMERMANN, WH;HOLMAN, RT
通讯作者：
HOLMAN, RT
影响因子：
2
作者：
Kangani, Cyrous O.;Kelley, David E.;Evans, Rhobert W.
通讯作者：
Evans, Rhobert W.
DOI：
10.1016/j.jchromb.2007.12.002
发表时间：
2008-02-01
影响因子：
3
作者：
Parise, Robert A.;Beumer, Jan H.;Egorin, Merrill J.
通讯作者：
Egorin, Merrill J.