Non-destructive two-photon excited fluorescence imaging identifies early nodules in calcific aortic-valve disease.
Non-destructive two-photon excited fluorescence imaging identifies early nodules in calcific aortic-valve disease.
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DOI：
10.1038/s41551-017-0152-3
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发表时间：
2017-11
影响因子：
28.1
通讯作者：
Georgakoudi I
中科院分区：
文献类型：
--
作者：
Baugh LM;Liu Z;Quinn KP;Osseiran S;Evans CL;Huggins GS;Hinds PW;Black LD 3rd;Georgakoudi I
在健康骨骼发育过程中，以及在钙化性主动脉瓣疾病（CAVD）和许多其他疾病发病时会发生钙化。尽管调节早期钙沉积的机制尚未完全清楚，但它们可能为新的治疗方法和早期干预提供靶点。在此，我们表明双光子激发荧光（TPEF）能够对钙化结节的形成提供定量且灵敏的检测结果，特别是在CAVD的情况下。具体而言，通过对来自切除的人类CAVD瓣膜以及脱矿前后的大鼠骨骼，还有在工程凝胶内形成的钙化结节的TPEF光谱图像进行分解，我们确定了一种内源性荧光团，它与样本中的矿化程度相关。然后我们开发了一种比率成像方法，该方法能够对早期钙化中矿物质沉积的存在进行定量检测。TPEF应该能够对三维组织样本进行无损、高分辨率成像，以评估钙化的存在。
Calcifications occur during the development of healthy bone, and at the onset of calcific aortic-valve disease (CAVD) and many other pathologies. Although the mechanisms regulating early calcium deposition are not fully understood, they may provide targets for new treatments and for early interventions. Here, we show that two-photon excited fluorescence (TPEF) can provide quantitative and sensitive readouts of calcific nodule formation, in particular in the context of CAVD. Specifically, by means of the decomposition of TPEF spectral images from excised human CAVD valves and from rat bone prior to and following demineralization, as well as from calcific nodules formed within engineered gels, we identified an endogenous fluorophore that correlates with the level of mineralization in the samples. We then developed a ratiometric imaging approach that provides a quantitative readout of the presence of mineral deposits in early calcifications. TPEF should enable non-destructive, high-resolution imaging of three-dimensional tissue specimens for the assessment of the presence of calcification.
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影响因子：
2.6
作者：
Guerraty, Marie;Mohler, Emile R., III
通讯作者：
Mohler, Emile R., III
DOI：
10.2174/1874210601004010223
发表时间：
2010-12-15
期刊：
The open dentistry journal
影响因子：
--
作者：
Cho A;Suzuki S;Hatakeyama J;Haruyama N;Kulkarni AB
通讯作者：
Kulkarni AB
影响因子：
2.7
作者：
Gerson, Cindy J.;Goldstein, Steven;Heacox, Albert E.
通讯作者：
Heacox, Albert E.
影响因子：
5
作者：
Hassan, T.;Piton, N.;Thiberville, L.
通讯作者：
Thiberville, L.
影响因子：
3.5
作者：
Fu, Henry L.;Leng, Yuxin;Li, Xingde
通讯作者：
Li, Xingde