Effect of perinatal hypothyroidism on the developmental regulation of rat pituitary growth hormone and thyrotropin genes.
Effect of perinatal hypothyroidism on the developmental regulation of rat pituitary growth hormone and thyrotropin genes.
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围产期甲状腺功能减退症对大鼠垂体生长激素和促甲状腺素基因发育调控的影响。
DOI：
10.1210/endo.136.10.7664653
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发表时间：
1995
期刊：
影响因子：
4.8
通讯作者：
A. Pérez
中科院分区：
文献类型：
--
作者：
M. Rodríguez;T. Jolín;A. Santos;A. Pérez
本文研究了甲状腺激素(T3)对大鼠垂体发育过程中生长激素(GH)、促甲状腺激素(TSH)和T3受体(TR)基因表达及脱碘酶活性的影响。逆转录-聚合酶链式反应检测到胎龄第15天GH和TSHβ基因的表达。虽然有一定的差异，但GH和TSHβ基因的表达在胎儿和新生儿时期均处于T3调控之下。在整个研究期间，对照动物和甲状腺功能减退动物之间观察到血浆中TSH浓度的差异，但没有观察到脑垂体中的TSH浓度。Trα和trβ基因在胎儿脑垂体中均有表达。Trα1、trβ2和c-erbaα2转录本表现出与trβ1不同的发育特征。甲状腺激素抑制trα1、trβ2和c-erbaα2，刺激trβ1。I型和II型脱碘酶活性(分别为5‘DI和5’DII)具有不同的个体发生模式；5‘D-II是胎儿的主要脱碘酶，水平与成人相似，而5’D-I水平较低，并随年龄增加而增加。T3刺激5‘D-I，降低5’D-II。这些结果表明，在促生长激素和促甲状腺激素细胞中，负责T3作用的机制在发育早期就已经成熟和活跃，表明这种激素参与了促生长激素和促甲状腺激素表型的建立和/或维持。
We studied the effects of thyroid hormone (T3) on GH, TSH, and T3 receptor (TR) gene expression as well as deiodinase activities during rat pituitary development. By reverse transcriptase-polymerase chain reaction, GH and TSH beta transcripts were detectable on fetal day 15. Although with certain differences, the expression of both GH and TSH beta genes was under T3 control during fetal and neonatal life. Differences in plasma, but not pituitary, TSH concentrations were observed between control and hypothyroid animals throughout the period studied. Both TR alpha and TR beta genes were expressed in the fetal pituitary. TR alpha 1, TR beta 2, and c-erbA alpha 2 transcripts displayed a developmental profile different from that of TR beta 1. Thyroid hormone repressed TR alpha 1, TR beta 2, and c-erbA alpha 2 and stimulated TR beta 1. Type I and type II deiodinase activities (5'DI and 5'DII, respectively) had different ontogenic patterns; 5'D-II was the predominant activity in fetuses, with levels similar to those in adults, whereas the level of 5'D-I was low and increased with age. T3 stimulated 5'D-I and decreases 5'D-II. These results demonstrate that in somatotroph and thyrotroph cells, the mechanisms responsible for T3 action are mature and active very early in development and suggest an involvement of this hormone in the establishment and/or maintenance of the somatotroph and thyrotroph phenotype.
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DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Godine,JE;Chin,WW;Habener,JF
通讯作者：
Habener,JF
两种不同甲状腺激素受体的大鼠 cDNA 克隆的分离和表征。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Murray,MB;Zilz,ND;McCreary,NL;MacDonald,MJ;Towle,HC
通讯作者：
Towle,HC
影响因子：
20.3
作者：
M. Berry;P. R. Larsen
通讯作者：
M. Berry;P. R. Larsen
大鼠生长激素基因的 5-侧翼 DNA 介导甲状腺激素的调节表达。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Casanova,J;Copp,RP;Janocko,L;Samuels,HH
通讯作者：
Samuels,HH
大鼠棕色脂肪组织中碘甲状腺原氨酸 5-脱碘酶的激素调节。
DOI：
10.1152/ajpendo.1986.251.6.e639
发表时间：
1986
期刊：
The American journal of physiology
影响因子：
--
作者：
Silva,JE;Larsen,PR
通讯作者：
Larsen,PR