Molecular basis of coronary artery dilation and aneurysms in patients with Kawasaki disease based on differential protein expression.
Molecular basis of coronary artery dilation and aneurysms in patients with Kawasaki disease based on differential protein expression.
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基于差异蛋白表达的川崎病患者冠状动脉扩张和动脉瘤的分子基础
DOI：
10.3892/mmr.2017.8111
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发表时间：
2018-03
影响因子：
3.4
通讯作者：
Jia H
中科院分区：
文献类型：
--
作者：
Liu W;Liu C;Zhang L;Xie X;Gu X;Sang C;Xu M;Xu W;Jia H
川崎病(KD)是一种儿童获得性心脏病，发病率高。KD有多种并发症，包括冠状动脉扩张(CAD)和冠状动脉瘤(CAA)。鉴定差异表达的蛋白质和潜在的机制可能是理解这些KD并发症之间差异的关键。在这项研究中，使用相对和绝对定量的等压标记来识别KD合并CAD和CAA患者之间的血清蛋白变化。在对照样本(健康人)与KD患者和CAD或CAA患者之间的比较中，总共鉴定出87个(37个上调，50个下调)和65个(33个上调，32个下调)显著差异表达的蛋白质。对潜在生物学过程的研究表明，这两种并发症之间的差异与伤口愈合反应以及脂蛋白和胆固醇相关的过程有关。利用ClueGo和ReactomeFIViz软件，通过丰富的生物学过程和途径分析，鉴定了参与伤口愈合信号网络形成的重要蛋白质。本研究筛选了5个差异表达蛋白，包括甘露糖结合凝集素2(MBL2)、补体因子H(CFH)、激肽原1(KNG1)、丝氨酸C家族成员1(SERPINC1)和纤维连接蛋白1(FN1)。分析表明，这些蛋白质与免疫、炎症和新陈代谢有关，在每个模块中发挥关键作用，这是以前从未报道过的。本研究提出MBL2、CFH、KNG1、SERPINC1和FN1可能是区分冠心病和CAA两大KD并发症的良好指标。
Kawasaki disease (KD) is an acquired cardiac disease with a high incidence that affects children. KD has various complications, including coronary artery dilation (CAD) and coronary artery aneurysms (CAA). The identification of differentially expressed proteins and the underlying mechanisms may be the key to understanding differences between these KD complications. In the present study, isobaric tags for relative and absolute quantitation were used to identify variations in serum proteins between KD patients with CAD and CAA. In total, 87 (37 upregulated and 50 downregulated) and 65 (33 upregulated and 32 downregulated) significantly differentially-expressed proteins were identified in comparisons between control samples (healthy individuals) and those obtained from patients with KD and with CAD or CAA. Investigation into the underlying biological process revealed that variations between the two complications were associated with the wound healing response, as well as lipoprotein- and cholesterol-associated processes. Important proteins involved in the formation of the wound healing signaling network were identified via enriched biological processes and pathway analysis using ClueGo and ReactomeFIViz software. In the present study, 5 significantly differentially-expressed proteins, including mannose binding lectin 2 (MBL2), complement factor H (CFH), kininogen 1 (KNG1), serpin family C member 1 (SERPINC1) and fibronectin 1 (FN1), were selected and confirmed by western blotting. Analysis indicated that these proteins were associated to immunity, inflammation and metabolism, serving a key role within each module, which has never been reported previously. The present study proposed that MBL2, CFH, KNG1, SERPINC1 and FN1 may be a potentially excellent indicator group for distinguishing the two major KD complications, CAD and CAA.
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影响因子：
3.7
作者：
Brunel H;Massanet R;Martinez-Perez A;Ziyatdinov A;Martin-Fernandez L;Souto JC;Perera A;Soria JM
通讯作者：
Soria JM
影响因子：
2.1
作者：
Watanabe T
通讯作者：
Watanabe T
影响因子：
7.3
作者：
Perkins SJ;Fung KW;Khan S
通讯作者：
Khan S
影响因子：
5.7
作者：
Simioni, Paolo;Tormene, Daniela;Prandoni, Paolo
通讯作者：
Prandoni, Paolo
DOI：
10.1093/bioinformatics/btp101
发表时间：
2009-04-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Bindea G;Mlecnik B;Hackl H;Charoentong P;Tosolini M;Kirilovsky A;Fridman WH;Pagès F;Trajanoski Z;Galon J
通讯作者：
Galon J