Genomic and phenotypic characterization of Vibrio cholerae non-O1 isolates from a US Gulf Coast cholera outbreak.
Genomic and phenotypic characterization of Vibrio cholerae non-O1 isolates from a US Gulf Coast cholera outbreak.
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DOI：
10.1371/journal.pone.0086264
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Colwell RR
中科院分区：
文献类型：
--
作者：
Haley BJ;Choi SY;Grim CJ;Onifade TJ;Cinar HN;Tall BD;Taviani E;Hasan NA;Abdullah AH;Carter L;Sahu SN;Kothary MH;Chen A;Baker R;Hutchinson R;Blackmore C;Cebula TA;Huq A;Colwell RR
2010年11月至2011年5月期间，记录了11例霍乱病例，与伊斯帕尼奥拉岛同时爆发的疫情无关，病原体霍乱弧菌血清组O75被追溯到从佛罗里达州阿巴拉契科拉湾收获的牡蛎。从11例确诊病例中分离出8株霍乱弧菌，并对其基因组进行了测序。基因组分析表明，存在一组先前被证明参与霍乱疾病过程的可移动元件（ctxAB、VPI-1和-2以及一种VSP-II样变体），系统基因组分析表明，这些分离物是23年前分离的一种临床菌株产毒霍乱弧菌V51血清群O141的姐妹类群。在美国东南部已多次从临床病例中分离出产毒霍乱弧菌O75，几十年来在全球从临床病例中分离出产毒霍乱弧菌O141。对分离株进行了比较基因组学、表型分析和秀丽隐杆线虫感染模型。这一分析加上O75和O141霍乱弧菌的分离数据表明，这些菌株可能是造成全球重大疾病负担的引起霍乱的菌株的一个未被充分认识的分支。
Between November 2010, and May 2011, eleven cases of cholera, unrelated to a concurrent outbreak on the island of Hispaniola, were recorded, and the causative agent, Vibrio cholerae serogroup O75, was traced to oysters harvested from Apalachicola Bay, Florida. From the 11 diagnosed cases, eight isolates of V. cholerae were isolated and their genomes were sequenced. Genomic analysis demonstrated the presence of a suite of mobile elements previously shown to be involved in the disease process of cholera (ctxAB, VPI-1 and -2, and a VSP-II like variant) and a phylogenomic analysis showed the isolates to be sister taxa to toxigenic V. cholerae V51 serogroup O141, a clinical strain isolated 23 years earlier. Toxigenic V. cholerae O75 has been repeatedly isolated from clinical cases in the southeastern United States and toxigenic V. cholerae O141 isolates have been isolated globally from clinical cases over several decades. Comparative genomics, phenotypic analyses, and a Caenorhabditis elegans model of infection for the isolates were conducted. This analysis coupled with isolation data of V. cholerae O75 and O141 suggests these strains may represent an underappreciated clade of cholera-causing strains responsible for significant disease burden globally.
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影响因子：
4.2
作者：
Haley BJ;Grim CJ;Hasan NA;Choi SY;Chun J;Brettin TS;Bruce DC;Challacombe JF;Detter JC;Han CS;Huq A;Colwell RR
通讯作者：
Colwell RR
影响因子：
3.8
作者：
Marin MA;Thompson CC;Freitas FS;Fonseca EL;Aboderin AO;Zailani SB;Quartey NK;Okeke IN;Vicente AC
通讯作者：
Vicente AC
影响因子：
4.4
作者：
Halpern, M;Gancz, H;Kashi, Y
通讯作者：
Kashi, Y
DOI：
10.1073/pnas.1207359109
发表时间：
2012-07-17
影响因子：
11.1
作者：
Hasan, Nur A.;Choi, Seon Young;Colwell, Rita R.
通讯作者：
Colwell, Rita R.
DOI：
10.1016/j.diagmicrobio.2005.06.020
发表时间：
2006-01-01
影响因子：
2.9
作者：
Lukinmaa, S;Mattila, K;Siitonen, A
通讯作者：
Siitonen, A