Chronic exposure to warm temperature causes low sperm abundance and quality in Drosophila melanogaster.
Chronic exposure to warm temperature causes low sperm abundance and quality in Drosophila melanogaster.
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DOI：
10.1038/s41598-023-39360-7
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发表时间：
2023-07-30
影响因子：
4.6
通讯作者：
Drummond-Barbosa, Daniela
中科院分区：
文献类型：
--
作者：
Gandara, Ana Caroline P.;Drummond-Barbosa, Daniela
温度影响整个生物体的雄性生育能力；然而，次优温度如何影响成人精子发生仍有待研究。在最近一项关于果蝇卵子发生的研究中，我们观察到暴露于温暖温度（29°C）的成年雄性果蝇的生育能力急剧下降。在这里，我们发现男性在 29°C 时会因为精子丰度和质量低而变得不育。 29°C 时精子丰度较低并非源于生殖系干细胞或精子细胞数量减少，因为这些数量在 29°C 和对照 25°C 之间保持可比。值得注意的是，在寒冷的 18°C 和 29°C 下，雄性精子细胞伸长和个体化缺陷的频率类似地增加，考虑到在 18°C 下测量的高精子丰度和雄性生育力，表明精子发生对伸长和个体化缺陷具有高度耐受性。有趣的是，在 29°C 时，精子的丰度突然下降，并且在精子发生快结束时转变为精囊时没有细胞凋亡的证据，这表明精子通过未知的机制被消除。最后，在 29°C 的温度下，男性的精子使卵子受精的效率较低，并且无法支持胚胎发生第一阶段之后的胚胎，这表明精子质量差是 29°C 下男性不育的另一个原因。
Temperature influences male fertility across organisms; however, how suboptimal temperatures affect adult spermatogenesis remains understudied. In a recent study on Drosophila melanogaster oogenesis, we observed a drastic reduction in the fertility of adult males exposed to warm temperature (29 °C). Here, we show that males become infertile at 29 °C because of low sperm abundance and quality. The low sperm abundance at 29 °C does not stem from reduced germline stem cell or spermatid numbers, as those numbers remain comparable between 29 °C and control 25 °C. Notably, males at cold 18 °C and 29 °C had similarly increased frequencies of spermatid elongation and individualization defects which, considering the high sperm abundance and male fertility measured at 18 °C, indicate that spermatogenesis has a high tolerance for elongation and individualization defects. Interestingly, the abundance of sperm at 29 °C decreases abruptly and with no evidence of apoptosis as they transition into the seminal vesicle near the end of spermatogenesis, pointing to sperm elimination through an unknown mechanism. Finally, sperm from males at 29 °C fertilize eggs less efficiently and do not support embryos past the first stage of embryogenesis, indicating that poor sperm quality is an additional cause of male infertility at 29 °C.
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DOI：
10.1080/21565562.2015.1006089
发表时间：
2015-01
期刊：
Spermatogenesis
影响因子：
--
作者：
Ben-David G;Miller E;Steinhauer J
通讯作者：
Steinhauer J
影响因子：
11.8
作者：
Arama, E;Agapite, J;Steller, H
通讯作者：
Steller, H
影响因子：
1.6
作者：
FRANKEL, AWK;PETERS, U;MEYER, GF
通讯作者：
MEYER, GF
影响因子：
10.5
作者：
FREEMAN, M;GLOVER, DM
通讯作者：
GLOVER, DM
影响因子：
56.9
作者：
DOANE, WW
通讯作者：
DOANE, WW