Risk of Illness with Salmonella due to Consumption of Raw Unwashed Vegetables Irrigated with Water from the Bogotá River
Risk of Illness with Salmonella due to Consumption of Raw Unwashed Vegetables Irrigated with Water from the Bogotá River
复制标题
食用波哥大河水灌溉的未清洗的生蔬菜导致沙门氏菌患病的风险
作者：
Laura X. Henao‐Herreño;A. M. López;J. P. Ramos;C. Haas;J. Husserl
博戈塔河接收来自博戈塔市和许多其他城镇的未经处理的废水。在博戈塔的下游，河水被用来灌溉农作物。河流中指示生物的浓度很高，这与粪便污染一致。为了调查由于暴露于来自河流的肠道病原体（特别是沙门氏菌）而导致疾病的可能性，我们在一个地区的六个采样点从博戈塔河采集了水样，该地区未经处理的河水用于灌溉生菜、西兰花和卷心菜。通过直接分离和qPCR定量沙门氏菌浓度。浓度不同，取决于所用的定量技术，qPCR和直接分离的范围分别为107.7和109.9基因invA拷贝数/L以及105.3和108.4 CFU/L。使用沙门氏菌浓度数据构建了一个定量微生物风险评估模型，该模型估计了因食用用博戈塔河水灌溉的生未洗蔬菜而导致沙门氏菌疾病的每日风险。根据qPCR估计的浓度，食用生的未清洗蔬菜的每日患病概率范围为0.62至0.85、0.64至0.86和0.64至0.85（基于通过直接分离估计的浓度，分别为0.47-0.85、0.47-0.86和0.41-0.85），这些都超过了通常提出的每年10 - 4的基准。在这项研究中获得的结果强调了在该地区进行适当的废水处理的必要性，并强调了收获后的做法，如清洗，消毒和烹饪的重要性。
The Bogotá River receives untreated wastewater from the city of Bogotá and many other towns. Downstream from Bogotá, water from the river is used for irrigation of crops. Concentrations of indicator organisms in the river are high, which is consistent with fecal contamination. To investigate the probability of illness due to exposure to enteric pathogens from the river, specifically Salmonella, we took water samples from the Bogotá River at six sampling locations in an area where untreated water from the river is used for irrigation of lettuce, broccoli, and cabbage. Salmonella concentrations were quantified by direct isolation and qPCR. Concentrations differed, depending on the quantification technique used, ranging between 107.7 and 109.9 number of copies of gene invA per L and 105.3 and 108.4 CFU/L, for qPCR and direct isolation, respectively. A quantitative microbial risk assessment model that estimates the daily risk of illness with Salmonella resulting from consuming raw unwashed vegetables irrigated with water from the Bogotá River was constructed using the Salmonella concentration data. The daily probability of illness from eating raw unwashed vegetables ranged between 0.62 and 0.85, 0.64 and 0.86, and 0.64 and 0.85 based on concentrations estimated by qPCR (0.47–0.85, 0.47–0.86, and 0.41–0.85 based on concentrations estimated by direct isolation) for lettuce, cabbage, and broccoli, respectively, which are all above the commonly propounded benchmark of 10−4 per year. Results obtained in this study highlight the necessity for appropriate wastewater treatment in the region, and emphasize the importance of postharvest practices, such as washing, disinfecting, and cooking.
对暴露于海滩沙子的人类疾病进行定量微生物风险评估。
影响因子：
11.4
作者：
Shibata,Tomoyuki;Solo-Gabriele,HelenaM
通讯作者：
Solo-Gabriele,HelenaM