Incorporating nanocrystalline cellulose into a multifunctional hydrogel for heart valve tissue engineering applications.
Incorporating nanocrystalline cellulose into a multifunctional hydrogel for heart valve tissue engineering applications.
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DOI：
10.1002/jbm.a.37267
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发表时间：
2022-01
影响因子：
4.9
通讯作者：
Butcher, Jonathan T.
中科院分区：
文献类型：
--
作者：
Ma, Nianfang;Cheung, Daniel Y.;Butcher, Jonathan T.
关键词：
近 30 年来，功能性组织工程心脏瓣膜 (TEHV) 一直是一个难以实现的目标。持续存在的挑战之一是对工程瓣叶的要求，该瓣叶具有非线性弹性组织生物力学特性、支持静止成纤维细胞表型并抵抗成骨分化。纳米纤维素是一种有吸引力的可调节生物材料，但尚未用于此应用。在这项研究中，我们通过将 TEMPO 改性的纳米晶纤维素 (mNCC) 缀合到甲基丙烯酸明胶 (MeGel) 的主链上，制备了一系列可光交联的复合水凝胶 mNCC-MeGel (mNG)。通过 FTIR、1HNMR 和单轴压缩测试对其结构进行了表征。人类脂肪源性间充质干细胞 (HADMSC) 被封装在材料内，并在正常和成骨培养基中培养 14 天后评估瓣膜间质细胞表型。与 MeGel 对照组相比，封装在 mNG 内的 HADMSC 显示α平滑肌肌动蛋白 (αSMA) 表达减少，波形蛋白和聚集蛋白聚糖表达增加，表明该材料支持静止的成纤维细胞表型。在成骨介质条件下，mNG 水凝胶内的 HADMSC 显示出较低的成骨基因表达，包括 Runx2 和骨钙素，表明对钙化的抵抗力。作为原理证明，mNG 水凝胶与粘度增强剂相结合，用于 3D 生物打印一个高的、自立的管状结构，以维持细胞活力。总之，这些结果表明 mNG 对于 TEHV 应用来说是一种有吸引力的生物材料。
Functional tissue engineered heart valves (TEHV) have been an elusive goal for nearly 30 years. Among the persistent challenges are the requirements for engineered valve leaflets that possess nonlinear elastic tissue biomechanical properties, support quiescent fibroblast phenotype, and resist osteogenic differentiation. Nanocellulose is an attractive tunable biological material that has not been employed to this application. In this study, we fabricated a series of photocrosslinkable composite hydrogels mNCC-MeGel (mNG) by conjugating TEMPO-modified nanocrystalline cellulose (mNCC) onto the backbone of methacrylated gelatin (MeGel). Their structures were characterized by FTIR, 1HNMR and uniaxial compression testing. Human adipose-derived mesenchymal stem cells (HADMSC) were encapsulated within the material and evaluated for valve interstitial cell phenotypes over 14 days culture in both normal and osteogenic media. Compared to the MeGel control group, the HADMSC encapsulated within mNG showed decreased alpha smooth muscle actin (αSMA) expression and increased vimentin and aggrecan expression, suggesting the material supports a quiescent fibroblastic phenotype. Under osteogenic media conditions, HADMSC within mNG hydrogels showed lower expression of osteogenic genes, including Runx2 and osteocalcin, indicating resistance towards calcification. As a proof of principle, the mNG hydrogel, combined with a viscosity enhancing agent, was used to 3D bioprint a tall, self-standing tubular structure that sustained cell viability. Together, these results identify mNG as an attractive biomaterial for TEHV applications.
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影响因子：
9
作者：
Hockaday LA;Kang KH;Colangelo NW;Cheung PY;Duan B;Malone E;Wu J;Girardi LN;Bonassar LJ;Lipson H;Chu CC;Butcher JT
通讯作者：
Butcher JT
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.9
作者：
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通讯作者：
BARRETT, AJ