A Simple Reversed Iontophoresis-Based Sensor to Enable In Vivo Multiplexed Measurement of Plant Biomarkers Using Screen-Printed Electrodes.
A Simple Reversed Iontophoresis-Based Sensor to Enable In Vivo Multiplexed Measurement of Plant Biomarkers Using Screen-Printed Electrodes.
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DOI：
10.3390/s23020780
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发表时间：
2023-01-10
期刊：
影响因子：
--
通讯作者：
Haseloff J
中科院分区：
文献类型：
--
作者：
Ruiz-Gonzalez A;Kempson H;Haseloff J
直接量化植物汁液中的生物标志物对提高作物产量至关重要。然而，目前的方法是不准确的，涉及非特异性参数的测量，如颜色强度的叶子，或需要高度侵入性的过程中提取汁液。此外，这些方法依赖于庞大且昂贵的设备，并且它们是耗时的。本工作报告的第一次低成本的传感装置，可用于同时测定汁液K+和pH值在活的植物通过反向离子电渗。通过沉积K+选择性膜来修饰丝网印刷电极，实现了70 mV Log[K+]−1的超能斯特灵敏度和微摩尔水平内的检测限。此外，通过电沉积RuOx颗粒来改性反向离子电渗装置的阴极材料。该电极可用于从植物叶片中直接提取离子和生理范围内（pH 3-8）的pH的安培测定，由RuOx与H+的选择性反应触发。此外，还设计了一种便携式和低成本（<60英镑）的基于微处理器的设备，使其能够在低资源环境中使用。该系统的适用性，证明了通过测量的K+和pH值在番茄植物中的浓度的变化之前和之后浇水与去离子水。这些结果代表了在设计用于监测植物中关键生物标志物的负担得起的非侵入性设备方面向前迈出了一步，在智能农业和精准农业等领域有大量应用。
The direct quantification of plant biomarkers in sap is crucial to enhancing crop production. However, current approaches are inaccurate, involving the measurement of non-specific parameters such as colour intensity of leaves, or requiring highly invasive processes for the extraction of sap. In addition, these methods rely on bulky and expensive equipment, and they are time-consuming. The present work reports for the first time a low-cost sensing device that can be used for the simultaneous determination of sap K+ and pH in living plants by means of reverse iontophoresis. A screen-printed electrode was modified by deposition of a K+-selective membrane, achieving a super-Nernstian sensitivity of 70 mV Log[K+]−1 and a limit of detection within the micromolar level. In addition, the cathode material of the reverse iontophoresis device was modified by electrodeposition of RuOx particles. This electrode could be used for the direct extraction of ions from plant leaves and the amperometric determination of pH within the physiological range (pH 3–8), triggered by the selective reaction of RuOx with H+. A portable and low-cost (<£60) microcontroller-based device was additionally designed to enable its use in low-resource settings. The applicability of this system was demonstrated by measuring the changes in concentration of K+ and pH in tomato plants before and after watering with deionised water. These results represent a step forward in the design of affordable and non-invasive devices for the monitoring of key biomarkers in plants, with a plethora of applications in smart farming and precision agriculture among others.
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影响因子：
5.1
作者：
Lowe A;Harrison N;French AP
通讯作者：
French AP
影响因子：
5.8
作者：
Diacci C;Abedi T;Lee JW;Gabrielsson EO;Berggren M;Simon DT;Niittylä T;Stavrinidou E
通讯作者：
Stavrinidou E
影响因子：
20.5
作者：
Pe単uelas, J;Filella, I
通讯作者：
Filella, I
影响因子：
3.7
作者：
Khan, Ayesha;Rashid, Audil;Chong, Ren
通讯作者：
Chong, Ren
DOI：
10.3390/s90604955
发表时间：
2009
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Kurzweil P
通讯作者：
Kurzweil P