Diurnal in vivo xylem sap glucose and sucrose monitoring using implantable organic electrochemical transistor sensors.
Diurnal in vivo xylem sap glucose and sucrose monitoring using implantable organic electrochemical transistor sensors.
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DOI：
10.1016/j.isci.2020.101966
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发表时间：
2021-01-22
期刊：
影响因子：
5.8
通讯作者：
Stavrinidou E
中科院分区：
文献类型：
--
作者：
Diacci C;Abedi T;Lee JW;Gabrielsson EO;Berggren M;Simon DT;Niittylä T;Stavrinidou E
将生化信号转换为电子读数的生物电子设备使得能够以高时空分辨率进行生物感测。这些技术主要应用于生物医学，而在植物中，传感主要基于需要组织采样的侵入性方法，阻碍了体内检测并且具有较差的时空分辨率。在这里，我们开发了基于有机电化学晶体管（OECTs）的酶生物传感器，用于体内和实时监测树木血管组织中的糖波动。将葡萄糖和蔗糖OECT生物传感器植入树木的血管组织中，并通过低成本便携式装置操作48小时。我们的工作包括一项概念验证研究，其中植入式OECT生物传感器不仅可以实时监测植物中的代谢物，还可以揭示昼夜糖稳态的新见解。我们预计，这项工作将有助于建立生物电子技术作为植物科学，农业和林业的强大的微创工具。在体内，使用OECTs实时监测48小时的树木中的糖波动OECTs揭示了白杨中先前未表征的昼夜蔗糖波动用于检测蔗糖的OECT的多酶功能化使用低成本便携式单元操作传感器生物技术;生物电子学;植物生理学
Bioelectronic devices that convert biochemical signals to electronic readout enable biosensing with high spatiotemporal resolution. These technologies have been primarily applied in biomedicine while in plants sensing is mainly based on invasive methods that require tissue sampling, hindering in-vivo detection and having poor spatiotemporal resolution. Here, we developed enzymatic biosensors based on organic electrochemical transistors (OECTs) for in-vivo and real-time monitoring of sugar fluctuations in the vascular tissue of trees. The glucose and sucrose OECT-biosensors were implanted into the vascular tissue of trees and were operated through a low-cost portable unit for 48hr. Our work consists a proof-of-concept study where implantable OECT-biosensors not only allow real-time monitoring of metabolites in plants but also reveal new insights into diurnal sugar homeostasis. We anticipate that this work will contribute to establishing bioelectronic technologies as powerful minimally invasive tools in plant science, agriculture and forestry. In vivo, real-time monitoring of sugars fluctuations in trees with OECTs for 48hr OECTs reveal previously uncharacterized diurnal sucrose fluctuations in aspen Multienzyme functionalization of OECT for detection of sucrose Operation of sensors with low-cost portable unit Biotechnology; Bioelectronics; Plant Physiology
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影响因子：
6.8
作者：
Diacci, Chiara;Lee, Jee Woong;Stavrinidou, Eleni
通讯作者：
Stavrinidou, Eleni
影响因子：
29.4
作者：
Liao, Caizhi;Mak, Chunhin;Yan, Feng
通讯作者：
Yan, Feng
影响因子：
6.9
作者：
Chaudhuri, Bhavna;Hoermann, Friederike;Frommer, Wolf B.
通讯作者：
Frommer, Wolf B.
影响因子：
4.6
作者：
Coppede, Nicola;Janni, Michela;Zappettini, Andrea
通讯作者：
Zappettini, Andrea
影响因子：
4
作者：
Daley, MJ;Phillips, NG
通讯作者：
Phillips, NG