Human CD14dim monocytes patrol and sense nucleic acids and viruses via TLR7 and TLR8 receptors.
Human CD14dim monocytes patrol and sense nucleic acids and viruses via TLR7 and TLR8 receptors.
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DOI：
10.1016/j.immuni.2010.08.012
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发表时间：
2010-09-24
期刊：
影响因子：
32.4
通讯作者：
Geissmann F
中科院分区：
文献类型：
--
作者：
Cros J;Cagnard N;Woollard K;Patey N;Zhang SY;Senechal B;Puel A;Biswas SK;Moshous D;Picard C;Jais JP;D'Cruz D;Casanova JL;Trouillet C;Geissmann F
单核细胞是对微生物的炎症反应的效应者。人类CD14+单核细胞擅长吞噬和产生活性氧物种，并在广泛的微生物信号作用下分泌炎性细胞因子。在这里，我们描述了缺乏CD14(CD14dim)和表达CD16的单核细胞的功能。CD14dim单核细胞在遗传上不同于自然杀伤细胞。基因表达分析表明，它们与巡逻小鼠的Gr1dim单核细胞相似，在过继移植后以淋巴细胞功能相关抗原1依赖的方式巡逻血管内皮细胞。CD14dim单核细胞为弱吞噬细胞，不产生细胞表面Toll样受体反应的ROS或细胞因子。相反，它们通过TLR7-TLR8-MYD88-MEK途径选择性地产生肿瘤坏死因子-α、白介素1-β和CCL3，以响应病毒和含有核酸的免疫复合体。因此，CD14dim细胞是真正的单核细胞，参与组织的天然局部监测和自身免疫性疾病的发病机制。►人CD14dim单核细胞巡视血管系统►CD14dim单核细胞对TLR1TLR2和TLR4激动剂►CD14DIM单核细胞通过TLR7-8-MYD88-MEK途径对病毒和核酸的反应很差
Monocytes are effectors of the inflammatory response to microbes. Human CD14+ monocytes specialize in phagocytosis and production of reactive oxygen species and secrete inflammatory cytokines in response to a broad range of microbial cues. Here, we have characterized the functions of human monocytes that lack CD14 (CD14dim) and express CD16. CD14dim monocytes were genetically distinct from natural killer cells. Gene expression analyses indicated similarities with murine patrolling Gr1dim monocytes, and they patrolled the endothelium of blood vessels after adoptive transfer, in a lymphocyte function-associated antigen-1-dependent manner. CD14dim monocytes were weak phagocytes and did not produce ROS or cytokines in response to cell-surface Toll-like receptors. Instead, they selectively produced TNF-α, IL-1β, and CCL3 in response to viruses and immune complexes containing nucleic acids, via a proinflammatory TLR7-TLR 8-MyD88-MEK pathway. Thus, CD14dim cells are bona fide monocytes involved in the innate local surveillance of tissues and the pathogenesis of autoimmune diseases. ► Human CD14dim monocytes patrol the vasculature ► CD14dim monocytes respond poorly to TLR1, TLR2, and TLR4 agonists ► CD14 dim monocytes respond to viruses and nucleic acids via TLR7-8-MyD88-MEK pathway
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影响因子：
3.7
作者：
Ancuta, P;Kuntsman, KJ;Gabuzda, D
通讯作者：
Gabuzda, D
影响因子：
20.3
作者：
Levy, Ofer;Suter, Eugenie E.;Wessels, Michael R.
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Wessels, Michael R.
影响因子：
4.4
作者：
Ancuta P;Liu KY;Misra V;Wacleche VS;Gosselin A;Zhou X;Gabuzda D
通讯作者：
Gabuzda D
影响因子：
4.4
作者：
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通讯作者：
Pulendran, Bali
影响因子：
19.6
作者：
Hill, GS;Delahousse, M;Bariéty, J
通讯作者：
Bariéty, J