Bone marrow microenvironmental changes underlie reduced RAG-mediated recombination and B cell generation in aged mice.
Bone marrow microenvironmental changes underlie reduced RAG-mediated recombination and B cell generation in aged mice.
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DOI：
10.1084/jem.20040845
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发表时间：
2004-08-16
影响因子：
15.3
通讯作者：
Gerstein, RM
中科院分区：
文献类型：
--
作者：
Labrie, JE;Sah, AP;Allman, DM;Cancro, MP;Gerstein, RM
During aging, adaptive immunity is severely compromised, due in part to decreased production of B lymphocytes and loss of immunoglobulin (Ig) diversity. However, the molecular mechanisms that underlie age-associated diminished B cell production remain unclear. Using in vivo labeling, we find that this reduction in marrow pre–B cells reflects increased attrition during passage from the pro–B to pre–B cell pool. Analyses of reciprocal bone marrow chimeras reveal that the magnitude and production rates of pre–B cells are controlled primarily by microenvironmental factors, rather than intrinsic events. To understand changes in pro–B cells that could diminish production of pre–B cells, we evaluated rag2 expression and V(D)J recombinase activity in pro–B cells at the single cell level. The percentage of pro–B cells that express rag2 is reduced in aged mice and is correlated with both a loss of V(D)J recombinase activity in pro–B cells and reduced numbers of pre–B cells. Reciprocal bone marrow chimeras revealed that the aged microenvironment also determines rag2 expression and recombinase activity in pro–B cells. Together, these observations suggest that extrinsic factors in the bone marrow that decline with age are largely responsible for less efficient V(D)J recombination in pro–B cells and diminished progression to the pre–B cell stage.
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影响因子：
4.4
作者：
Miller, JP;Allman, D
通讯作者：
Allman, D
DOI：
10.1073/pnas.0237043100
发表时间：
2003-02-04
影响因子：
11.1
作者：
Akamatsu, Y;Monroe, R;Oettinger, MA
通讯作者：
Oettinger, MA
影响因子：
56.9
作者：
OETTINGER, MA;SCHATZ, DG;BALTIMORE, D
通讯作者：
BALTIMORE, D
影响因子：
15.3
作者：
Borghesi, L;Hsu, LY;Miller, JP;Anderson, M;Herzenberg, L;Herzenberg, L;Schlissel, MS;Allman, D;Gerstein, RM
通讯作者：
Gerstein, RM
影响因子：
32.4
作者：
Hsu, LY;Lauring, J;Schlissel, MS
通讯作者：
Schlissel, MS