Cloning and sequence analysis of the M gene of porcine epidemic diarrhea virus LJB/03.
Cloning and sequence analysis of the M gene of porcine epidemic diarrhea virus LJB/03.
复制标题
猪流行腹泻病毒LJB/03的M基因的克隆和序列分析。
DOI：
10.1007/s11262-004-4583-z
复制
发表时间：
2005-01
期刊：
影响因子：
1.6
通讯作者：
Yijing L
中科院分区：
文献类型：
--
作者：
Jinghui F;Yijing L
从黑龙江省某猪场感染猪流行性腹泻病毒（PEDV）的仔猪粪便中分离到一株PEDV LJB/03。从粪便中直接提取RNA，通过RT-PCR扩增出LJB/03的M基因，并将其克隆到pMD 18-T载体中。对M基因cDNA进行测序，其包含681个核苷酸的开放阅读框，编码226个氨基酸的蛋白质。LJB/03 M基因具有152个腺嘌呤（22%）、153个胞嘧啶（23%）、161个鸟嘌呤（24%）和214个胸腺嘧啶（31%）的碱基组成。序列分析表明，LJB/03 M基因与其它PEDV分离株的同源性分别为：JMe 2 97.80%，KPEDV-9（韩国野毒株）96.92%，KPEDV-9（韩国株）97.36%，Br 1/87 97.80%，CV 777 97.94%。与CV 777、Br 1/87、JMe 2、KPEDV-9、KPEDV-9的氨基酸同源性分别为97.79%、97.35%、96.90%、96.46%。M基因的序列分析，包括遗传距离测量，系统发育树分析和残基替换分析，表明所有其他PED病毒分析分为三组，LJB/03本身分支成一个独立的组。结果表明，LJB/03株M基因核苷酸序列与其它PED病毒相比存在一定的变异。
Porcine epidemic diarrhea virus (PEDV) LJB/03 was isolated from the fece of piglets infected with PEDV on a pig farm, Heilongjiang province, China. The M gene of LJB/03 was amplified from the RNA extracted directly from the fece samples by RT-PCR and cloned into pMD18-T vector. The M gene cDNA was sequenced and encompasses an open reading frame of 681 nucleotides, encoding a 226-amino acid protein. The LJB/03 M gene has a base composition of 152 adenines (22%), 153 cytosines (23%), 161 guanines (24%), and 214 thymines (31%). Sequence comparison with other PEDV strains selected from GenBank revealed that the LJB/03 M gene has a high sequence homology to those of other PEDV isolates, 97.80% with JMe2, 96.92% with KPEDV-9 (Korean field isolate), 97.36% with KPEDV-9 (Korean), 97.80% with Br1/87, and 97.94% with CV777. The encoded protein shared 97.79% amino acid identities compared with CV777, 97.35% with Br1/87, 97.79% with JMe2, 96.90% with KPEDV-9 (Korean field isolate), 96.46% with KPEDV-9 (Korean). Sequence analysis of the M gene, including genetic distance measurement, phylogenetic tree analysis, and residue substitution analysis, showed that all other PED viruses analyzed fell into three groups, and the LJB/03 itself branched in an independent group. These data revealed that the M gene nucleotide sequence of LJB/03 has some mutations in comparison with the other PED viruses.
猪流行腹泻病毒Chinju99的尖峰基因的克隆和序列分析。
影响因子：
1.6
作者：
Yeo SG;Hernandez M;Krell PJ;Nagy EE
通讯作者：
Nagy EE
影响因子：
2.2
作者：
PIJPERS, A;VANNIEUWSTADT, AP;VERHEIJDEN, JHM
通讯作者：
VERHEIJDEN, JHM
影响因子：
3.3
作者：
Knuchel M;Ackermann M;Müller HK;Kihm U
通讯作者：
Kihm U